Intermittent tensile strain induces an increased response in bone formation markers compared to continuous load in mouse pre-osteoblasts when loading magnitude is matched

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Scott, R ORCID: https://orcid.org/0000-0001-7642-545X, Varley, I ORCID: https://orcid.org/0000-0002-3607-8921, Sale, C, Tarum, J ORCID: https://orcid.org/0009-0007-1690-7191, James, R ORCID: https://orcid.org/0000-0002-7119-3159, Barnett, CT ORCID: https://orcid.org/0000-0001-6898-9095 and Santos, L ORCID: https://orcid.org/0000-0002-1915-6780, 2024. Intermittent tensile strain induces an increased response in bone formation markers compared to continuous load in mouse pre-osteoblasts when loading magnitude is matched. Journal of the Mechanical Behavior of Biomedical Materials, 159: 106683. ISSN 1751-6161

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Official URL: https://doi.org/10.1016/j.jmbbm.2024.106683

Abstract

Intermittent and continuous mechanical loads are known to influence osteogenic activity. The present study examines the effects of matched intermittent and continuous load in vitro on bone formation markers. MC3T3 (mouse pre-osteoblasts) were cultured and placed in a bioreactor to undergo continuous, intermittent, or unloading for 1, 3 and 12 days. Loading conditions were matched for magnitude, duration and frequency. Each time point was analysed for alkaline phosphatase (ALP) activity, procollagen 1 N-terminal propeptide (PINP) and alizarin red staining (ARS). Intermittent load caused an increase in ALP activity across all time points compared to continuous loading (↑30%–59%) and unloaded conditions (↑70%–90%). PINP concentrations from intermittent load were lower than continuous load (↓112%) on day 3. However, no differences were observed in PINP concentrations between loading conditions at other time points. No differences were observed for ARS between loading conditions. Intermittent load caused an increase in bone formation marker ALP, but not PINP, when compared to continuous loading and unloaded conditions. These findings further our knowledge in bone formation response and provide additional tools for the analysis of osteogenesis in vitro.

Item Type:	Journal article
Publication Title:	Journal of the Mechanical Behavior of Biomedical Materials
Creators:	Scott, R., Varley, I., Sale, C., Tarum, J., James, R., Barnett, C.T. and Santos, L.
Publisher:	Elsevier
Date:	November 2024
Volume:	159
ISSN:	1751-6161
Identifiers:	Number Type 10.1016/j.jmbbm.2024.106683 DOI S1751616124003151 Publisher Item Identifier 2202117 Other
Rights:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	28 Aug 2024 09:42
Last Modified:	28 Aug 2024 09:42
URI:	https://irep.ntu.ac.uk/id/eprint/52110