SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures

Marthandan, S., Freeburn, R., Steinbrecht, S., Pawelec, G. and Barnett, Y., 2014. SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures. Immunology and Ageing, 11. ISSN 1742-4933

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Abstract

Background: Damage to T cells of the immune system by reactive oxygen species may result in altered cell function or cell death and thereby potentially impact upon the efficacy of a subsequent immune response. Here, we assess the impact of the antioxidants Ebselen and N-acetyl cysteine on a range of biological markers in human T cells derived from a SENIEUR status donor. In addition, the impact of these antioxidants on different MAP kinase pathways in T cells from donors of different ages was also examined. Methods: T cell clones were derived from healthy 26, 45 and SENIEUR status 80 year old people and the impact of titrated concentrations of Ebselen or N-acetyl cysteine on their proliferation and in vitro lifespan, GSH:GSSG ratio as well as levels of oxidative DNA damage and on MAP kinase signaling pathways was examined.

Item Type: Journal article
Publication Title: Immunology and Ageing
Creators: Marthandan, S., Freeburn, R., Steinbrecht, S., Pawelec, G. and Barnett, Y.
Publisher: BioMed Central
Date: 2014
Volume: 11
ISSN: 1742-4933
Identifiers:
NumberType
10.1186/s12979-014-0017-5DOI
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 09:58
Last Modified: 23 Aug 2016 09:07
URI: http://irep.ntu.ac.uk/id/eprint/5782

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