Hyperinvasiveness in the major food-borne pathogen Campylobacter jejuni

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Javed, MA, 2009. Hyperinvasiveness in the major food-borne pathogen Campylobacter jejuni. PhD, Nottingham Trent University.

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Abstract

Campylobacter jejuni is a common cause of human gastrointestinal infections. Invasion of host epithelial cells is believed to be an important virulence Mechanism of this bacterium. C. jejuni strains vary in their ability to invade the human epithelial cells and some of the strains are hyperinvasive. The aim of this work was to find the molecular basis of this hyperinvasive phenotype. The previously studied hyperinvasive phenotype of C. jejuni 01/51 in INT-407 cells was verified using Caco-2 cell based invasion assay and the assay was set up at Nottingham Trent University by selecting blood agar for 48 hours as the pre-assay bacterial growth conditions and 100 multiplicity of infection as the starting inoculum. Seven hundred and sixty eight mutants generated in this strain by random transposon insertional mutagenesis were screened for their ability to invade human intestinal epithelial cells in an in vitro model and 174 mutants were selected for further studies. The motility of selected mutants was determined and 40 mutants that showed more than 75% motility compared to wildtype strain, with a very low level of invasion were selected for reconfirmation of this reduced invasion phenotype using standard INT-407 and Caco-2 cells based invasion assays. Localisation of the transposon insertion site by plasmid rescue was attempted in 15 mutants that showed very low levels of invasion in both eukaryotic cell lines.

Item Type:	Thesis
Creators:	Javed, M.A.
Date:	2009
Rights:	This work is the intellectual property of the author, and may also be owned by the research sponsor(s) and/ or Nottingham Trent University. You may copy up to 5 % of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the first instance to the author.
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 09:34
Last Modified:	09 Oct 2015 09:34
URI:	https://irep.ntu.ac.uk/id/eprint/141