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Sachana, M., Flaskos, J. and Hargreaves, A.J. 
ORCID: 0000-0001-9754-5477,
2005.
Effects of chlorpyrifos and chlorpyrifos-methyl on the outgrowth of axon-like processes, tubulin, and GAP-43 in N2a cells.
Toxicology Mechanisms and Methods, 15 (6), pp. 405-410.
ISSN 1537-6524
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Abstract
The aim of this work was to study the neurodegenerative effects of the organophosphate (OP) pesticides chlorpyrifos (CPF) and chlorpyrifos-methyl (CHM) on cultured mouse N2a neuroblastoma cells. CPF or CHM, at a subcytotoxic concentration of 3 μM, were added to the cells either at the time of the induction of cell differentiation (codifferentiation) or 16 h after the induction of differentiation (postdifferentiation). CPF and CHM were similar in inhibiting significantly the outgrowth of axon-like processes from N2a cells after only 4 h exposure under both co- and postdifferentiation exposure conditions. Densitometric scanning of Western blots of extracts of cells treated with CPF or CHM for 4 h revealed significantly decreased cross-reactivity with a monoclonal antibody recognizing the protein GAP-43 under post- but not under codifferentiation exposure conditions. Exposure to CPF or CHM for 4 h under postdifferentiation conditions also resulted in reduced fluorescence of N2a cell body staining with anti-GAP-43. Cross-reactivity of Western blots with a monoclonal antibody recognizing -tubulin was not significantly affected by OP treatment. These data indicate that a disturbance in GAP-43 may be important in the retraction of axons in predifferentiated N2a cells and support the notion that the mechanisms involved in CPF-and CHM-induced inhibition of axonal outgrowth may be different under co- and postdifferentiation exposure conditions.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Toxicology Mechanisms and Methods | ||||
| Creators: | Sachana, M., Flaskos, J. and Hargreaves, A.J. | ||||
| Publisher: | Taylor & Francis (Routledge) | ||||
| Date: | 2005 | ||||
| Volume: | 15 | ||||
| Number: | 6 | ||||
| ISSN: | 1537-6524 | ||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | EPrints Services | ||||
| Date Added: | 09 Oct 2015 10:35 | ||||
| Last Modified: | 09 Jun 2017 13:33 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/15080 |
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