Towards an understanding of peptide-inorganic interactions

Limo, MJ, 2013. Towards an understanding of peptide-inorganic interactions. PhD, Nottingham Trent University.

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Abstract

This study has contributed to the developing understanding of fundamental principles through which interactions at peptide-inorganic interfaces occur. The inorganic materials; crystalline zinc oxide (ZnO) and platinum (Pt) together with specific binding peptides identified using the phage display technique and alanine scanning for mutant sequences selected on the basis of peptide stability calculated in silico were synthesized, extensively characterized and the mechanisms of their interaction and the effects thereof studied. Firstly ZnO growth was monitored during solution synthesis from precursors using two different ZnO methods in the absence and presence of ZnO binding peptides (ZnO-BPs); G-12 (GLHVMHKVAPPR), its mutants (G-12A6, G-12A11, G-12A12) and GT-16 (GLHVMHKVAPPR-GGGC). Secondly, adsorption characteristics and thermodynamics of interaction of ZnO with ZnO-BPs and Pt with platinum binding peptides (Pt-BPs) were studied using biophysical tools; quartz crystal microbalance with dissipation monitoring (QCM-D) and isothermal titration calorimetry (ITC).

Item Type:	Thesis
Creators:	Limo, M.J.
Date:	2013
Rights:	This work is the intellectual property of the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the first instance to the owner of the Intellectual Property Rights
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 09:35
Last Modified:	09 Oct 2015 09:35
URI:	https://irep.ntu.ac.uk/id/eprint/216

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