The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells

Tools

Sachana, M., Flaskos, J., Alexaki, E., Glynn, P. and Hargreaves, A.J. ORCID: 0000-0001-9754-5477, 2001. The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells. Toxicology in Vitro, 15 (4), pp. 369-372. ISSN 0887-2333

Preview

Text
188082_5709 Hargreaves PrePrint.pdf
Download (86kB) | Preview

Official URL: http://www.elsevier.com/wps/find/journaldescriptio...

Abstract

The aim of this work was to study the effects of chlorpyrifos (CPF) on the outgrowth of axons by differentiating mouse N2a neuroblastoma cells. This was achieved by morphological, Western blotting and enzymatic analyses of cells induced to differentiate in the presence and absence of CPF added either at the same time (co-differentiation) or 16 h after (post-differentiation) the induction of cell differentiation. The outgrowth of axon-like processes was impaired following 4 or 8 h exposure to CPF in both co- and post-differentiation experiments. Western blotting analysis revealed reduced levels of neurofilament heavy chain (NF-H) following 8 h of exposure but no significant effect at 4 h under both co- and post-differentiation conditions. By contrast, levels of the heat shock protein HSP-70 were raised at both time points, but only in co-differentiation experiments. Neuropathy target esterase (NTE) activity was lower than controls following 4 or 8 h of exposure under co-differentiation conditions, but not under any post-differentiation conditions. The results suggest that the inhibition of axon production and maintenance by CPF in differentiating N2a cells may involve multiple targets, which are different under co- and post-differentiation conditions.

Item Type:

Journal article

Publication Title:

Toxicology in Vitro

Creators:

Sachana, M., Flaskos, J., Alexaki, E., Glynn, P. and Hargreaves, A.J.

Publisher:

Elsevier (not including Cell Press)

Place of Publication:

Oxford

Date:

2001