Investigating the potential of the Metastasis Associated Antigen 1 (MTA1) for cancer immunotherapy in a murine model

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Assudani, D, 2007. Investigating the potential of the Metastasis Associated Antigen 1 (MTA1) for cancer immunotherapy in a murine model. PhD, Nottingham Trent University.

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Abstract

Immunotherapeutic approaches to target antigens associated with metastasis could provide a valuable means of targeting metastatic cells specifically. Metastasis Associated Antigen (MTA1) is one such relatively novel antigen, which has been associated with aggressive tumours, and shown to be over expressed in breast, oesophageal, colorectal, gastric and pancreatic cancer, amongst others. Various studies have indicated that MTA1 is essential for the transformation of cells and hence targeting it is unlikely to generate antigen loss variants. This study proposed to investigate MTA1 as a potential target for immunotherapy in a murine tumour model. We have shown that murine MTA1 (mMTA1) mRNA is highly expressed in most of the tumour cell lines as compared to normal tissues, which express mMTA1 at very low levels. Furthermore, to rule out any post-transcriptional modifications, MTA1 protein levels were also confirmed by western blotting. It was observed that most of the cell lines expressed MTA1 at high levels, whereas no protein expression was detected in the normal tissues by western blotting. Next, we decided to identify MHC class I and II restricted immunogenic peptides from murine and human MTA1 gene for syngeneic and transgenic mice respectively.

Item Type:	Thesis
Creators:	Assudani, D.
Date:	2007
Rights:	This work is the intellectual property of the author, and may also be owned by the research sponsor(s) and/or Nottingham Trent University. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, of if a more substantial copy is required, should be directed in the first instance to the author.
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 09:35
Last Modified:	09 Oct 2015 09:35
URI:	https://irep.ntu.ac.uk/id/eprint/230