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Chaturvedi, N, Kajsik, M, Forsythe, S 
ORCID: https://orcid.org/0000-0002-6709-0712 and Pandey, PN,
2015.
Protein sequences insight into heavy metal tolerance in Cronobacter sakazakii BAA-894 encoded by plasmid pESA3.
Archives of Microbiology, 197 (10), pp. 1141-1149.
ISSN 0302-8933
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Abstract
The recently annotated genome of the bacterium Cronobacter sakazakii BAA-894 suggests the organism has the ability to bind heavy metals. This study demonstrates heavy metal tolerance in Cronobacter sakazakii, in which proteins with the heavy metal interaction were recognized by computational and experimental study. As the result, approximately one
fourth of proteins encoded on the plasmid pESA3 are proposed to have potential interaction with heavy metals. Interaction between heavy metals and predicted proteins was further
corroborated using protein crystal structures from protein data bank database and comparison of metal-binding ligands. In addition with, a phylogenetic study was undertaken for most toxic heavy metals, like arsenic, cadmium, lead and mercury and obtained related tree pattern for lead, cadmium and arsenic. Laboratory studies confirmed the organism's tolerance to tellurite, copper and silver. These experimental and computational study data extend our
understanding of the genes encoding for proteins of this important neonatal pathogen and provides further insights into the genotypes associated with features that can contribute to its persistence in the environment. The information will be of value for future environmental
protection from heavy toxic metals.
| Item Type: | Journal article |
|---|---|
| Publication Title: | Archives of Microbiology |
| Creators: | Chaturvedi, N., Kajsik, M., Forsythe, S. and Pandey, P.N. |
| Publisher: | Springer |
| Date: | December 2015 |
| Volume: | 197 |
| Number: | 10 |
| ISSN: | 0302-8933 |
| Identifiers: | Number Type 10.1007/s00203-015-1147-7 DOI |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Jonathan Gallacher |
| Date Added: | 04 Nov 2015 16:33 |
| Last Modified: | 09 Jun 2017 13:56 |
| URI: | https://irep.ntu.ac.uk/id/eprint/26127 |
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