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Bajuszova, Z, Ali, Z, Scott, SM, Seetohul, LN 
ORCID: https://orcid.org/0000-0001-6261-0259 and Islam, M,
2016.
Cavity enhanced immunoassay measurements in microtiter plates using BBCEAS.
Analytical Chemistry, 88 (10), pp. 5264-5270.
ISSN 0003-2700
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Abstract
We report on the first detailed use of broadband cavity enhanced absorption spectroscopy (BBCEAS) as a detection system for immunoassay. A vertical R ≥ 0.99 optical cavity was integrated with a motorised XY stage, which functioned as a receptacle for 96 well microtiter plates. The custom built cavity enhanced microplate reader was used to make measurements on a commercially available osteocalcin sandwich ELISA kit. A 30 fold increase in path length was obtained with a minimum detectable change in the absorption coefficient, αmin(t), of 5.3 × 10-5 cm-1 Hz-1/2. This corresponded to a 39 fold increase in the sensitivity of measurement when directly compared to measurements in a conventional microplate reader. Separate measurements of a standard STREP-HRP colorimetric reaction in microtiter plates of differing optical quality produced an increase in sensitivity of up to 115 fold compared to a conventional microplate reader. The sensitivity of the developed setup compared favorably with previous liquid-phase cavity enhanced studies and approaches the sensitivity of typical fluorometric ELISAs. It could benefit any biochemical test which uses single pass absorption as a detection method, through either the label free detection of biologically important molecules at lower concentrations or the reduction in the amount of expensive biochemicals needed for a particular test, leading to cheaper tests.
| Item Type: | Journal article |
|---|---|
| Publication Title: | Analytical Chemistry |
| Creators: | Bajuszova, Z., Ali, Z., Scott, S.M., Seetohul, L.N. and Islam, M. |
| Publisher: | ACS (American Chemical Society) Publications |
| Date: | 18 April 2016 |
| Volume: | 88 |
| Number: | 10 |
| ISSN: | 0003-2700 |
| Identifiers: | Number Type 10.1021/acs.analchem.6b00375 DOI |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Jill Tomkinson |
| Date Added: | 26 Apr 2016 13:17 |
| Last Modified: | 13 Oct 2017 10:52 |
| URI: | https://irep.ntu.ac.uk/id/eprint/27689 |
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