Markedly different gene expression in wheat grown with organic or inorganic fertilizer

Lu, C ORCID: https://orcid.org/0000-0002-0064-4725, Hawkesford, MJ, Barraclough, PB, Poulton, PR, Wilson, ID, Barker, GL and Edwards, KJ, 2005. Markedly different gene expression in wheat grown with organic or inorganic fertilizer. Proceedings of the Royal Society B: Biological Sciences, 272 (1575), pp. 1901-1908. ISSN 0962-8452

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Abstract

Nitrogen is the major determinant of crop yield and quality and the precise management of nitrogen fertilizer is an important issue for farmers and environmentalists. Despite this, little is known at the level of gene expression about the response of field crops to different amounts and forms of nitrogen fertilizer. Here we use expressed sequence tag (EST)-based wheat microarrays in combination with the oldest continuously running agricultural experiment in the world to show that gene expression is significantly influenced by the amount and form of nitrogenous fertilizer. In the Broadbalk winter wheat experiment at Rothamsted in the United Kingdom and at three other diverse test sites, we show that specific genes have surprisingly different expression levels in the grain endosperm when nitrogen is supplied either in an organic or an inorganic form. Many of the genes showing differential expression are known to participate in nitrogen metabolism and storage protein synthesis. However, others are of unknown function and therefore represent new leads for future investigation. Our observations show that specific gene expression is diagnostic for use of organic sources of nitrogen fertilizer and may therefore have useful applications in defining the differences between organically and conventionally grown wheat.

Item Type:	Journal article
Publication Title:	Proceedings of the Royal Society B: Biological Sciences
Creators:	Lu, C., Hawkesford, M.J., Barraclough, P.B., Poulton, P.R., Wilson, I.D., Barker, G.L. and Edwards, K.J.
Publisher:	The Royal Society
Date:	22 September 2005
Volume:	272
Number:	1575
ISSN:	0962-8452
Identifiers:	Number Type 10.1098/rspb.2005.3161 DOI
Divisions:	Schools > School of Animal, Rural and Environmental Sciences
Record created by:	Linda Sullivan
Date Added:	08 Sep 2016 11:07
Last Modified:	09 Jun 2017 14:05
URI:	https://irep.ntu.ac.uk/id/eprint/28420

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