Actin nucleation by WH2 domains at the autophagosome

Coutts, AS ORCID: https://orcid.org/0000-0002-5005-1864 and La Thangue, NB, 2015. Actin nucleation by WH2 domains at the autophagosome. Nature Communications, 6, p. 7888. ISSN 2041-1723

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Abstract

Autophagy is a catabolic process whereby cytosolic components and organelles are degraded to recycle key cellular materials. It is a constitutive process required for proper tissue homoeostasis but can be rapidly regulated by a variety of stimuli (for example, nutrient starvation and chemotherapeutic agents). JMY is a DNA damage-responsive p53 cofactor and actin nucleator important for cell survival and motility. Here we show that JMY regulates autophagy through its actin nucleation activity. JMY contains an LC3-interacting region, which is necessary to target JMY to the autophagosome where it enhances the autophagy maturation process. In autophagosomes, the integrity of the WH2 domains allows JMY to promote actin nucleation, which is required for efficient autophagosome formation. Thus our results establish a direct role for actin nucleation mediated by WH2 domain proteins that reside at the autophagosome.

Item Type:	Journal article
Publication Title:	Nature Communications
Creators:	Coutts, A.S. and La Thangue, N.B.
Publisher:	Nature Publishing Group
Date:	July 2015
Volume:	6
ISSN:	2041-1723
Identifiers:	Number Type 10.1038/ncomms8888 DOI ncomms8888 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	21 Sep 2016 10:52
Last Modified:	13 Oct 2017 15:45
URI:	https://irep.ntu.ac.uk/id/eprint/28592

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