The transcriptional repressor Blimp1 is expressed in rare luminal progenitors and is essential for mammary gland development

Ahmed, MI ORCID: https://orcid.org/0000-0002-9051-7681, Elias, S, Mould, AW, Bikoff, EK and Robertson, EJ, 2016. The transcriptional repressor Blimp1 is expressed in rare luminal progenitors and is essential for mammary gland development. Development, 143 (10), pp. 1663-1673. ISSN 0950-1991

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Abstract

Mammary gland morphogenesis depends on a tight balance between cell proliferation, differentiation and apoptosis, to create a defined functional hierarchy within the epithelia. The limited availability of stem cell/progenitor markers has made it challenging to decipher lineage relationships. Here, we identify a rare subset of luminal progenitors that express the zinc finger transcriptional repressor Blimp1, and demonstrate that this subset of highly clonogenic luminal progenitors is required for mammary gland development. Conditional inactivation experiments using K14-Cre and WAPi-Cre deleter strains revealed essential functions at multiple developmental stages. Thus, Blimp1 regulates proliferation, apoptosis and alveolar cell maturation during puberty and pregnancy. Loss of Blimp1 disrupts epithelial architecture and lumen formation both in vivo and in three-dimensional (3D) primary cell cultures. Collectively, these results demonstrate that Blimp1 is required to maintain a highly proliferative luminal subset necessary for mammary gland development and homeostasis.

Item Type:	Journal article
Publication Title:	Development
Creators:	Ahmed, M.I., Elias, S., Mould, A.W., Bikoff, E.K. and Robertson, E.J.
Publisher:	The Company of Biologists Ltd
Date:	December 2016
Volume:	143
Number:	10
ISSN:	0950-1991
Identifiers:	Number Type 10.1242/dev.136358 DOI 646699 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	22 Aug 2017 07:44
Last Modified:	26 Oct 2020 15:11
URI:	https://irep.ntu.ac.uk/id/eprint/31456

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