Quantifying the efficiency of hydroxyapatite mineralising peptides

Plowright, R, Belton, DJ, Kaplan, DL and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2017. Quantifying the efficiency of hydroxyapatite mineralising peptides. Scientific Reports, 7, p. 7681. ISSN 2045-2322

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Abstract

We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N=10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors.

Item Type:	Journal article
Publication Title:	Scientific Reports
Creators:	Plowright, R., Belton, D.J., Kaplan, D.L. and Perry, C.C.
Publisher:	Nature Publishing Group
Date:	9 August 2017
Volume:	7
ISSN:	2045-2322
Identifiers:	Number Type 10.1038/s41598-017-07247-z DOI 7247 Publisher Item Identifier
Rights:	This article is licensed under a Creative Commons Attribution 4.0 International License.
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	27 Nov 2017 09:34
Last Modified:	27 Nov 2017 09:34
URI:	https://irep.ntu.ac.uk/id/eprint/32083

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