Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

Gillies, D, Gamal, W, Downes, A, Reinwald, Y ORCID: https://orcid.org/0000-0001-6733-605X, Yang, Y, El Haj, AJ and Bagnaninchi, PO, 2017. Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy. Methods. ISSN 1046-2023

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Abstract

Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed.
We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using Optical Coherence Phase Microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by cytochalasin D.
We showed the potential of qualitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.

Item Type:	Journal article
Publication Title:	Methods
Creators:	Gillies, D., Gamal, W., Downes, A., Reinwald, Y., Yang, Y., El Haj, A.J. and Bagnaninchi, P.O.
Publisher:	Elsevier
Date:	31 October 2017
ISSN:	1046-2023
Identifiers:	Number Type 10.1016/j.ymeth.2017.10.010 DOI
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	11 Dec 2017 12:05
Last Modified:	31 Oct 2018 03:00
URI:	https://irep.ntu.ac.uk/id/eprint/32179

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