Elevated fetal adipsin/acylation-stimulating protein (ASP) in obese pregnancy: novel placental secretion via Hofbauer cells

Sivakumar, K, Bari, MF, Adaikalakoteswari, A ORCID: https://orcid.org/0000-0003-2974-3388, Guller, S, Weickert, MO, Randeva, HS, Grammatopoulos, DK, Bastie, CC and Vatish, M, 2013. Elevated fetal adipsin/acylation-stimulating protein (ASP) in obese pregnancy: novel placental secretion via Hofbauer cells. The Journal of Clinical Endocrinology & Metabolism, 98 (10), pp. 4113-4122. ISSN 0021-972X

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Abstract

Context and Objective: Obesity in pregnancy is associated with increased risks of obesity in the offspring. We investigated the relationship between obesity in pregnancy and circulating maternal and fetal levels of adipose tissue-derived factors adipsin and acylation stimulating protein (ASP) in lean and obese mothers.

Design: Paired peripheral and cord blood samples were taken. Paired fat and placenta tissue were taken for explant culture. Media were assayed for secreted adipsin and ASP. Clinical parameters assayed included fasting insulin, glucose, and adipsin.

Setting: The study was conducted at a university hospital maternity unit.

Patients: Patients included 35 lean [body mass index (BMI) 19–25 kg/m2, mean age 32 years and 39 obese (BMI) > 30 kg/m2, mean age 32.49 years] pregnant Caucasian women, delivered by cesarean section at term.

Main Outcome Measure: Identification of placental macrophages [Hofbauer cells (HBCs)], as a source of adipsin and ASP was determined.

Results: HBCs secreted both adipsin and ASP. Cord levels of adipsin (1663.78 ± 52.76 pg/mL) and ASP (354.48 ± 17.17 ng/mL) were significantly elevated in the offspring of obese mothers compared with their lean controls [1354.66 ± 33.87 pg/mL and 302.63 ± 14.98 ng/mL, respectively (P < .05 for both)]. Placentae from obese mothers released significantly more adipsin and ASP than placentae from lean mothers [546.0 ± 44 pg/mL·g vs 284.56 ± 43 pg/mL·g and 5485.75 ± 163.32 ng/mL·g vs 2399.16 ± 181.83 ng/mL·g, respectively (P < .05 for both)]. Circulating fetal adipsin and ASP positively correlated with maternal BMI (r = 0.611, P < .0001, and r = 0.391, P < .05, respectively). Fetal adipsin correlated positively with maternal (r = 0.482, P < .01) and fetal homeostasis model assessment of insulin resistance (r = 0.465, P < .01).

Conclusions: We demonstrate novel secretion of adipsin and ASP by placental HBCs.

Item Type:	Journal article
Publication Title:	The Journal of Clinical Endocrinology & Metabolism
Creators:	Sivakumar, K., Bari, M.F., Adaikalakoteswari, A., Guller, S., Weickert, M.O., Randeva, H.S., Grammatopoulos, D.K., Bastie, C.C. and Vatish, M.
Publisher:	Oxford University Press
Date:	1 October 2013
Volume:	98
Number:	10
ISSN:	0021-972X
Identifiers:	Number Type 10.1210/jc.2012-4293 DOI 23956345 PubMed ID PMC3790615 Other
Rights:	Copyright © 2013 by The Endocrine Society.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	26 Apr 2018 15:25
Last Modified:	26 Apr 2018 15:25
URI:	https://irep.ntu.ac.uk/id/eprint/33360

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