The anion study: effect of different crystalloid solutions on acid base balance, physiology, and survival in a rodent model of acute isovolaemic haemodilution

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Ekbal, NJ, Hennis, P ORCID: https://orcid.org/0000-0002-8216-998X, Dyson, A, Mythen, M, James, MFM and Singer, M, 2018. The anion study: effect of different crystalloid solutions on acid base balance, physiology, and survival in a rodent model of acute isovolaemic haemodilution. British Journal of Anaesthesia. ISSN 0007-0912

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Official URL: http://doi.org/10.1016/j.bja.2018.01.026

Abstract

Background: Commercially available crystalloid solutions used for volume replacement do not exactly match the balance of electrolytes found in plasma. Large volume administration may lead to electrolyte imbalance and potential harm. We hypothesised that haemodilution using solutions containing different anions would result in diverse biochemical effects, particularly on acid-base status, and different outcomes.

Methods: Anaesthetised, fluid-resuscitated, male Wistar rats underwent isovolaemic haemodilution by removal of 10% blood volume every 15 min, followed by replacement with one of three crystalloid solutions based on acetate, lactate, or chloride. Fluids were administered in a protocolised manner to achieve euvolaemia based on echocardiography-derived left ventrical volumetric measures. Removed blood was sampled for plasma ions, acid-base status, haemoglobin, and glucose. This cycle was repeated at 15-min intervals until death. The primary endpoint was change in plasma bicarbonate within each fluid group. Secondary endpoints included time to death and cardiac function.

Results: During haemodilution, chloride-treated rats showed significantly greater decreases in plasma bicarbonate and strong ion difference levels compared with acetate- and lactate-treated rats. Time to death, total volume of fluid administered: chloride group 56 (3) ml, lactate group 62 (3) ml, and acetate group 65 (3) ml; haemodynamic and tissue oxygenation changes were, however, similar between groups.

Conclusions: With progressive haemodilution, resuscitation with a chloride-based solution induced more acidosis compared with lactate- and acetate-based solutions, but outcomes were similar. No short-term impact was seen from hyperchloraemia in this model.

Item Type:	Journal article
Publication Title:	British Journal of Anaesthesia
Creators:	Ekbal, N.J., Hennis, P., Dyson, A., Mythen, M., James, M.F.M. and Singer, M.
Publisher:	Elsevier
Date:	21 March 2018
ISSN:	0007-0912
Identifiers:	Number Type 10.1016/j.bja.2018.01.026 DOI S0007091218300849 Publisher Item Identifier
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	30 Apr 2018 13:31
Last Modified:	30 Apr 2018 13:31
URI:	https://irep.ntu.ac.uk/id/eprint/33393