Association of telomere shortening with impaired glucose tolerance and diabetic macroangiopathy

Adaikalakoteswari, A ORCID: https://orcid.org/0000-0003-2974-3388, Balasubramanyam, M, Ravikumar, R, Deepa, R and Mohan, V, 2007. Association of telomere shortening with impaired glucose tolerance and diabetic macroangiopathy. Atherosclerosis, 195 (1), pp. 83-89. ISSN 0021-9150

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Abstract

Objective: Shortening of telomere length has been reported in several conditions including Type 2 diabetes and atherosclerosis. The aims of this study were (1) to assess whether telomere shortening occurs at the stage of pre-diabetes, i.e., impaired glucose tolerance (IGT) and (2) whether telomere shortening was greater in Type 2 diabetic subjects with atherosclerotic plaques.

Methods: Subjects with impaired glucose tolerance (IGT) (n = 30), non-diabetic control subjects (n = 30), Type 2 diabetic patients without (n = 30) and with atherosclerotic plaques (n = 30) were selected from the Chennai Urban Rural Epidemiology Study (CURES), an ongoing epidemiological population-based study. Southern-blot analysis was used to determine mean terminal restriction fragment (TRF) length, a measure of average telomere size, in leukocyte DNA. Levels of thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCO) and high sensitive C-reactive protein (hs-CRP) were measured by standard methodologies. Carotid intima-media thickness (IMT) was assessed by high resolution B-mode ultrasonography.

Results: The mean (±S.E.) TRF lengths were significantly lower in IGT subjects (6.97 ± 0.3 kb; p = 0.002) and lower still in Type 2 diabetic subjects without plaques (6.21 ± 0.2; p = 0.0001) and lowest in Type 2 diabetic subjects with atherosclerotic plaques (5.39 ± 0.2; p = 0.0001) when compared to control subjects (8.7 ± 0.5). In IGT subjects, TRF length was positively correlated to HDL cholesterol and negatively correlated to glycated hemoglobin (HbA1c), TBARS, PCO, HOMA-IR and IMT. In multiple linear regression analysis, presence of diabetes, HDL cholesterol and increased TBARS levels appear as significant determinants of telomere shortening.

Conclusion: Telomere shortening is seen even at the stage of IGT. Among subjects with Type 2 diabetes, those with atherosclerotic plaques had greater shortening of telomere length compared to those without plaques.

Item Type:	Journal article
Publication Title:	Atherosclerosis
Creators:	Adaikalakoteswari, A., Balasubramanyam, M., Ravikumar, R., Deepa, R. and Mohan, V.
Publisher:	Elsevier Ireland Ltd.
Date:	November 2007
Volume:	195
Number:	1
ISSN:	0021-9150
Identifiers:	Number Type 10.1016/j.atherosclerosis.2006.12.003 DOI S0021915006007490 Publisher Item Identifier
Rights:	Copyright © 2006 Elsevier Ireland Ltd. All rights reserved.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	04 May 2018 14:16
Last Modified:	04 May 2018 14:16
URI:	https://irep.ntu.ac.uk/id/eprint/33446

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