Curcumin-induced inhibition of cellular reactive oxygen species generation: novel therapeutic implications

Balasubramanyam, M, Adaikalakoteswari, A ORCID: https://orcid.org/0000-0003-2974-3388, Kumar, RS, Monickaraj, SF, Maheswari, JU and Mohan, V, 2003. Curcumin-induced inhibition of cellular reactive oxygen species generation: novel therapeutic implications. Journal of Biosciences, 28 (6), pp. 715-721. ISSN 0250-5991

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Abstract

There is evidence for increased levels of circulating reactive oxygen species (ROS) in diabetics, as indirectly inferred by the findings of increased lipid peroxidation and decreased antioxidant status. Direct measurements of intracellular generation of ROS using fluorescent dyes also demonstrate an association of oxidative stress with diabetes. Although phenolic compounds attenuate oxidative stress-related tissue damage, there are concerns over toxicity of synthetic phenolic antioxidants and this has considerably stimulated interest in investigating the role of natural phenolics in medicinal applications. Curcumin (the primary active principle in turmeric, Curcuma longa Linn.) has been claimed to represent a potential antioxidant and antiinflammatory agent with phytonutrient and bioprotective properties. However there are lack of molecular studies to demonstrate its cellular action and potential molecular targets. In this study the antioxidant effect of curcumin as a function of changes in cellular ROS generation was tested. Our results clearly demonstrate that curcumin abolished both phorbol-12 myristate-13 acetate (PMA) and thapsigargin-induced ROS generation in cells from control and diabetic subjects. The pattern of these ROS inhibitory effects as a function of dose-dependency suggests that curcumin mechanistically interferes with protein kinase C (PKC) and calcium regulation. Simultaneous measurements of ROS and Ca2+ influx suggest that a rise in cytosolic Ca2+ may be a trigger for increased ROS generation. We suggest that the antioxidant and antiangeogenic actions of curcumin, as a mechanism of inhibition of Ca2+ entry and PKC activity, should be further exploited to develop suitable and novel drugs for the treatment of diabetic retinopathy and other diabetic complications.

Item Type:	Journal article
Publication Title:	Journal of Biosciences
Creators:	Balasubramanyam, M., Adaikalakoteswari, A., Kumar, R.S., Monickaraj, S.F., Maheswari, J.U. and Mohan, V.
Publisher:	Indian Academy of Sciences
Date:	December 2003
Volume:	28
Number:	6
ISSN:	0250-5991
Identifiers:	Number Type 10.1007/BF02708432 DOI 14660871 PubMed ID
Rights:	© Indian Academy of Sciences
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	14 May 2018 15:08
Last Modified:	14 May 2018 15:10
URI:	https://irep.ntu.ac.uk/id/eprint/33577

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