Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

Hadjidemetriou, M, Al-Ahmady, Z ORCID: https://orcid.org/0000-0001-7681-3735 and Kostarelos, K, 2016. Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles. Nanoscale, 8 (13), pp. 6948-6957. ISSN 2040-3364

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Abstract

Nanoparticles (NPs) are instantly modified once injected in the bloodstream because of their interaction with the blood components. The spontaneous coating of NPs by proteins, once in contact with biological fluids, has been termed the ‘protein corona’ and it is considered to be a determinant factor for the pharmacological, toxicological and therapeutic profile of NPs. Protein exposure time is thought to greatly influence the composition of protein corona, however the dynamics of protein interactions under realistic, in vivo conditions remain unexplored. The aim of this study was to quantitatively and qualitatively investigate the time evolution of in vivo protein corona, formed onto blood circulating, clinically used, PEGylated liposomal doxorubicin. Protein adsorption profiles were determined 10 min, 1 h and 3 h post-injection of liposomes into CD-1 mice. The results demonstrated that a complex protein corona was formed as early as 10 min post-injection. Even though the total amount of protein adsorbed did not significantly change over time, the fluctuation of protein abundances observed indicated highly dynamic protein binding kinetics.

Item Type:	Journal article
Publication Title:	Nanoscale
Creators:	Hadjidemetriou, M., Al-Ahmady, Z. and Kostarelos, K.
Publisher:	Royal Society of Chemistry
Date:	2016
Volume:	8
Number:	13
ISSN:	2040-3364
Identifiers:	Number Type 10.1039/C5NR09158F DOI
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	14 Nov 2018 11:10
Last Modified:	07 Dec 2018 15:34
URI:	https://irep.ntu.ac.uk/id/eprint/34963

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