Self-assembled mucin-containing microcarriers via hard templating on CaCO3 crystals

Balabushevich, NG, Sholina, EA, Mikhalchik, EV, Filatova, LY, Vikulina, AS ORCID: https://orcid.org/0000-0001-9427-2055 and Volodkin, D ORCID: https://orcid.org/0000-0001-7474-5329, 2018. Self-assembled mucin-containing microcarriers via hard templating on CaCO3 crystals. Micromachines, 9 (6), p. 307. ISSN 2072-666X

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Abstract

Porous vaterite crystals of CaCO3 are extensively used for the fabrication of self-assembled polymer-based microparticles (capsules, beads, etc.) utilized for drug delivery and controlled release. The nature of the polymer used plays a crucial role and discovery of new perspective biopolymers is essential to assemble microparticles with desired characteristics, such as biocompatibility, drug loading efficiency/capacity, release rate, and stability. Glycoprotein mucin is tested here as a good candidate to assemble the microparticles because of high charge due to sialic acids, mucoadhesive properties, and a tendency to self-assemble, forming gels. Mucin loading into the crystals via co-synthesis is twice as effective as via adsorption into preformed crystals. Desialylated mucin has weaker binding to the crystals most probably due to electrostatic interactions between sialic acids and calcium ions on the crystal surface. Improved loading of low-molecular-weight inhibitor aprotinin into the mucin-containing crystals is demonstrated. Multilayer capsules (mucin/protamine)3 have been made by the layer-by-layer self-assembly. Interestingly, the deposition of single mucin layers (mucin/water)3 has also been proven, however, the capsules were unstable, most probably due to additional (to hydrogen bonding) electrostatic interactions in the case of the two polymers used. Finally, approaches to load biologically-active compounds (BACs) into the mucin-containing microparticles are discussed.

Item Type:	Journal article
Publication Title:	Micromachines
Creators:	Balabushevich, N.G., Sholina, E.A., Mikhalchik, E.V., Filatova, L.Y., Vikulina, A.S. and Volodkin, D.
Publisher:	MDPI AG
Date:	2018
Volume:	9
Number:	6
ISSN:	2072-666X
Identifiers:	Number Type 10.3390/mi9060307 DOI mi9060307 Publisher Item Identifier
Rights:	© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	03 May 2019 08:23
Last Modified:	30 Jul 2019 14:35
URI:	https://irep.ntu.ac.uk/id/eprint/36403

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