Mucin adsorption on vaterite CaCO3 microcrystals for the prediction of mucoadhesive properties

Balabushevich, NG, Kovalenko, EA, Mikhalchik, EV, Filatova, LY, Volodkin, D ORCID: https://orcid.org/0000-0001-7474-5329 and Vikulina, AS ORCID: https://orcid.org/0000-0001-9427-2055, 2019. Mucin adsorption on vaterite CaCO3 microcrystals for the prediction of mucoadhesive properties. Journal of Colloid and Interface Science, 545, pp. 330-339. ISSN 0021-9797

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Abstract

Porous vaterite CaCO3 crystals are widely used as containers for drug loading and as sacrificial templates to assemble polymer-based nano- and micro-particles at mild conditions. Special attention is paid nowadays to mucosal delivery where the glycoprotein mucin plays a crucial role as a main component of a mucous. In this work mucoadhesive properties of vaterite crystals have been tested by investigation of mucin binding to the crystals as a function of (i) time, (ii) glycoprotein concentration, (iii) adsorption conditions and (iv) degree of mucin desialization. Mucin adsorption follows Bangham equation indicating that diffusion into crystal pores is the rate-limiting step. Mucin strongly binds to the crystals (ΔG = −35 ± 4 kJ mol−1) via electrostatic and hydrophobic interactions forming a gel and thus giving the tremendous mucin mass content in the crystals of up to 16%. Despite strong intermolecular mucin-mucin interactions, pure mucin spheres formed after crystal dissolution are unstable. However, introduction of protamine, actively used for mucosal delivery, makes the spheres stable via additional electrostatic bonding. The results of this work indicate that the vaterite crystals are extremely promising carriers for mucosal drug delivery and for development of test-systems for the analysis of the mucoadhesion.

Item Type:	Journal article
Publication Title:	Journal of Colloid and Interface Science
Creators:	Balabushevich, N.G., Kovalenko, E.A., Mikhalchik, E.V., Filatova, L.Y., Volodkin, D. and Vikulina, A.S.
Publisher:	Academic Press
Date:	2019
Volume:	545
ISSN:	0021-9797
Identifiers:	Number Type 10.1016/j.jcis.2019.03.042 DOI S0021979719303327 Publisher Item Identifier
Rights:	© 2019 the authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	03 May 2019 09:54
Last Modified:	30 Jul 2019 14:29
URI:	https://irep.ntu.ac.uk/id/eprint/36408

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