Ethylene glycol coated nanoceria protects against oxidative stress in human lens epithelium

Hanafy, BI, Cave, GWV ORCID: https://orcid.org/0000-0002-4167-1332, Barnett, Y ORCID: https://orcid.org/0000-0003-0271-2266 and Pierscionek, BK ORCID: https://orcid.org/0000-0002-8661-6353, 2019. Ethylene glycol coated nanoceria protects against oxidative stress in human lens epithelium. RSC Advances, 9 (29), pp. 16596-16605. ISSN 2046-2069

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Abstract

Chronic diseases are rising in incidence and prevalence because of increases in life expectancy in many parts of the world coupled with advances in medicine which manage disease progression, rather than curing and alleviating the causes. Cataract is one such chronic condition. Identifying a therapeutic intervention that is successful in reversing or preventing cataracts may have applications for other chronic diseases of protein misfolding, such as diabetes and Alzheimer's disease as these have similar causation factors, notably oxidative stress and/or glycation. Cerium oxide nanoparticles (nanoceria) which have antioxidant, radioprotective and enzyme-mimetic properties have the potential to lead to an effective non-surgical treatment. However, nanoceria stability in physiological media is poor thus hindering their effective use in biomedical applications. Here we report a highly efficient one-pot synthesis of nanoceria (2–5 nm) coated with ethylene glycol, that is colloidally stable in physiological media and exhibits multiwavelength photoluminescence. The formulation, up to concentrations of 200 μg ml−1, was not toxic to human lens epithelial cells and had no adverse effect on the cellular morphology or proliferation rate. More significantly, these nanoceria showed protective effects against oxidative stress induced by hydrogen peroxide in lens epithelial cells. Electron microscopy studies show the internalization and cytoplasmic localization of the nanoceria was found to be largely in the perinuclear region.

Item Type:	Journal article
Publication Title:	RSC Advances
Creators:	Hanafy, B.I., Cave, G.W.V., Barnett, Y. and Pierscionek, B.K.
Publisher:	Royal Society of Chemistry
Date:	17 May 2019
Volume:	9
Number:	29
ISSN:	2046-2069
Identifiers:	Number Type 10.1039/c9ra01252d DOI
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	29 May 2019 14:26
Last Modified:	29 May 2019 14:26
URI:	https://irep.ntu.ac.uk/id/eprint/36679

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