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Brosens, JJ, Salker, MS, Teklenburg, G, Nautiyal, J, Salter, S, Lucas, ES, Steel, JH, Christian, M 
ORCID: https://orcid.org/0000-0002-1616-4179, Chan, Y-W, Boomsma, CM, Moore, JD, Hartshorne, GM, Šućurović, S, Mulac-Jericevic, B, Heijnen, CJ, Quenby, S, Groot Koerkamp, MJ, Holstege, FCP, Shmygol, A and Macklon, NS,
2014.
Uterine selection of human embryos at implantation.
Scientific Reports, 4 (1): 3894.
ISSN 2045-2322
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Abstract
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in the decidualizing stroma before being rejected via mechanisms that are poorly understood. Here we show that developmentally impaired human embryos elicit an endoplasmic stress response in human decidual cells. A stress response was also evident upon in vivo exposure of mouse uteri to culture medium conditioned by low-quality human embryos. By contrast, signals emanating from developmentally competent embryos activated a focused gene network enriched in metabolic enzymes and implantation factors. We further show that trypsin, a serine protease released by pre-implantation embryos, elicits Ca2+ signaling in endometrial epithelial cells. Competent human embryos triggered short-lived oscillatory Ca2+ fluxes whereas low-quality embryos caused a heightened and prolonged Ca2+ response. Thus, distinct positive and negative mechanisms contribute to active selection of human embryos at implantation.
| Item Type: | Journal article |
|---|---|
| Publication Title: | Scientific Reports |
| Creators: | Brosens, J.J., Salker, M.S., Teklenburg, G., Nautiyal, J., Salter, S., Lucas, E.S., Steel, J.H., Christian, M., Chan, Y.-W., Boomsma, C.M., Moore, J.D., Hartshorne, G.M., Šućurović, S., Mulac-Jericevic, B., Heijnen, C.J., Quenby, S., Groot Koerkamp, M.J., Holstege, F.C.P., Shmygol, A. and Macklon, N.S. |
| Publisher: | Nature Publishing Group |
| Date: | 2014 |
| Volume: | 4 |
| Number: | 1 |
| ISSN: | 2045-2322 |
| Identifiers: | Number Type 10.1038/srep03894 DOI |
| Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Linda Sullivan |
| Date Added: | 08 Aug 2019 11:05 |
| Last Modified: | 08 Aug 2019 11:25 |
| URI: | https://irep.ntu.ac.uk/id/eprint/37193 |
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