The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid-binding amphipathic helix

Barneda, D, Planas-Iglesias, J, Gaspar, ML, Mohammadyani, D, Prasannan, S, Dormann, D, Han, G-S, Jesch, SA, Carman, GM, Kagan, V, Parker, MG, Ktistakis, NT, Klein-Seetharaman, J, Dixon, AM, Henry, SA and Christian, M ORCID: https://orcid.org/0000-0002-1616-4179, 2015. The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid-binding amphipathic helix. eLife, 4: e07485. ISSN 2050-084X

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Abstract

Maintenance of energy homeostasis depends on the highly regulated storage and release of triacylglycerol primarily in adipose tissue, and excessive storage is a feature of common metabolic disorders. CIDEA is a lipid droplet (LD)-protein enriched in brown adipocytes promoting the enlargement of LDs, which are dynamic, ubiquitous organelles specialized for storing neutral lipids. We demonstrate an essential role in this process for an amphipathic helix in CIDEA, which facilitates embedding in the LD phospholipid monolayer and binds phosphatidic acid (PA). LD pairs are docked by CIDEA trans-complexes through contributions of the N-terminal domain and a C-terminal dimerization region. These complexes, enriched at the LD–LD contact site, interact with the cone-shaped phospholipid PA and likely increase phospholipid barrier permeability, promoting LD fusion by transference of lipids. This physiological process is essential in adipocyte differentiation as well as serving to facilitate the tight coupling of lipolysis and lipogenesis in activated brown fat.

Item Type:	Journal article
Publication Title:	eLife
Creators:	Barneda, D., Planas-Iglesias, J., Gaspar, M.L., Mohammadyani, D., Prasannan, S., Dormann, D., Han, G.-S., Jesch, S.A., Carman, G.M., Kagan, V., Parker, M.G., Ktistakis, N.T., Klein-Seetharaman, J., Dixon, A.M., Henry, S.A. and Christian, M.
Publisher:	eLife Sciences
Date:	2015
Volume:	4
ISSN:	2050-084X
Identifiers:	Number Type 10.7554/elife.07485 DOI
Rights:	Copyright Barneda et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	08 Aug 2019 15:00
Last Modified:	08 Aug 2019 15:00
URI:	https://irep.ntu.ac.uk/id/eprint/37200

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