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Soroosh, A, Rankin, CR, Polytarchou, C 
ORCID: https://orcid.org/0000-0002-1948-7934, Lokhandwala, ZA, Patel, A, Chang, L, Pothoulakis, C, Iliopoulos, D and Padua, DM,
2019.
miR-24 is elevated in ulcerative colitis patients and regulates intestinal epithelial barrier function.
The American Journal of Pathology, 189 (9), pp. 1763-1774.
ISSN 0002-9440
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Abstract
Inflammatory bowel disease is characterized by high levels of inflammation and loss of barrier integrity in the colon. The intestinal barrier is a dynamic network of proteins that encircle intestinal epithelial cells. miRNAs regulate protein-coding genes. In this study, miR-24 was found to be elevated in colonic biopsies and blood samples from ulcerative colitis (UC) patients compared with healthy controls. In the colon of UC patients, miR-24 is localized to intestinal epithelial cells, which prompted an investigation of intestinal epithelial barrier function. Two intestinal epithelial cell lines were used to study the effect of miR-24 overexpression on barrier integrity. Overexpression of miR-24 in both cell lines led to diminished transepithelial electrical resistance and increased dextran flux, suggesting an effect on barrier integrity. Overexpression of miR-24 did not induce apoptosis or affect cell proliferation, suggesting that the effect of miR-24 on barrier function was due to an effect on cell–cell junctions. Although the tight junctions in cells overexpressing miR-24 appeared normal, miR-24 overexpression led to a decrease in the tight junction–associated protein cingulin. Loss of cingulin compromised barrier formation; cingulin levels negatively correlated with disease severity in UC patients. Together, these data suggest that miR-24 is a significant regulator of intestinal barrier that may be important in the pathogenesis of UC.
| Item Type: | Journal article |
|---|---|
| Publication Title: | The American Journal of Pathology |
| Creators: | Soroosh, A., Rankin, C.R., Polytarchou, C., Lokhandwala, Z.A., Patel, A., Chang, L., Pothoulakis, C., Iliopoulos, D. and Padua, D.M. |
| Publisher: | Elsevier |
| Date: | September 2019 |
| Volume: | 189 |
| Number: | 9 |
| ISSN: | 0002-9440 |
| Identifiers: | Number Type 10.1016/j.ajpath.2019.05.018 DOI S0002944019300896 Publisher Item Identifier |
| Rights: | © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Linda Sullivan |
| Date Added: | 30 Aug 2019 08:33 |
| Last Modified: | 30 Aug 2019 08:33 |
| URI: | https://irep.ntu.ac.uk/id/eprint/37520 |
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