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Dorling, JL, Clayton, DJ 
ORCID: https://orcid.org/0000-0001-5481-0891, Jones, J, Carter, WG, Thackray, AE, King, JA, Pucci, A, Batterham, RL and Stensel, DJ,
2019.
A randomized crossover trial assessing the effects of acute exercise on appetite, circulating ghrelin concentrations, and butyrylcholinesterase activity in normal-weight males with variants of the obesity-linked FTO rs9939609 polymorphism.
The American Journal of Clinical Nutrition, 110 (5), pp. 1055-1066.
ISSN 0002-9165
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Abstract
Background: The fat mass and obesity-associated gene (FTO) rs9939609 A-allele is associated with higher acyl-ghrelin (AG) concentrations, higher energy intake and obesity, though exercise may mitigate rs9939609 A-allele linked obesity risk. Butyrylcholinesterase (BChE) hydrolyses AG to des-acyl-ghrelin (DAG), potentially decreasing appetite. However, the effects of the FTO rs9939609 genotype and exercise on BChE activity, AG, DAG and energy intake are unknown.
Objective: We hypothesized that individuals homozygous for the obesity-risk A-allele (AAs) would exhibit higher postprandial AG and energy intake than individuals homozygous for the low obesity-risk T-allele (TTs), but that exercise would increase BChE activity and diminish these differences.
Methods: Twelve AA and 12 TT normal weight males completed a control (8 hours rest) and an exercise (1 hour of exercise at 70% peak oxygen uptake, 7 hours rest) trial in a randomized cross-over design. A fixed meal was consumed at 1.5 hours and an ab libitum buffet meal at 6.5 hours. Appetite, appetite-related hormones, BChE activity and energy intake were assessed.
Results: AAs displayed lower baseline BChE activity, higher baseline AG/DAG ratio, attenuated AG suppression after a fixed meal and higher ad libitum energy intake than TTs (ES ≥ 0.76, P ≤ 0.049). Exercise increased delta BChE activity in both genotypes (ES = 0.41, P = 0.004); however, exercise lowered AG and the AG/DAG ratio to a greater extent in AAs (P ≤ 0.041), offsetting the higher AG ghrelin profile observed in AAs during the control trial (ES ≥ 0.88, P ≤ 0.048). Exercise did not elevate energy intake in either genotype (P = 0.282).
Conclusions: Exercise increases BChE activity, suppresses AG and the AG/DAG ratio and corrects the higher AG profile observed in obesity-risk AA individuals. These findings suggest that exercise or other methods targeting BChE activity may offer a preventative and/or therapeutic strategy for AA individuals.
| Item Type: | Journal article |
|---|---|
| Alternative Title: | Ghrelin, exercise and FTO rs9939609 genotype [short running title] |
| Publication Title: | The American Journal of Clinical Nutrition |
| Creators: | Dorling, J.L., Clayton, D.J., Jones, J., Carter, W.G., Thackray, A.E., King, J.A., Pucci, A., Batterham, R.L. and Stensel, D.J. |
| Publisher: | Oxford University Press |
| Date: | November 2019 |
| Volume: | 110 |
| Number: | 5 |
| ISSN: | 0002-9165 |
| Identifiers: | Number Type 10.1093/ajcn/nqz188 DOI |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Linda Sullivan |
| Date Added: | 30 Aug 2019 09:09 |
| Last Modified: | 31 May 2021 15:07 |
| URI: | https://irep.ntu.ac.uk/id/eprint/37522 |
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