Multipotential stromal cell abundance in cellular bone allograft: comparison with fresh age-matched iliac crest bone and bone marrow aspirate

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Baboolal, TG, Boxall, SA, El-Sherbiny, YM ORCID: https://orcid.org/0000-0003-4791-3475, Moseley, TA, Cuthbert, RJ, Giannoudis, PV, McGonagle, D and Jones, E, 2014. Multipotential stromal cell abundance in cellular bone allograft: comparison with fresh age-matched iliac crest bone and bone marrow aspirate. Regenerative Medicine, 9 (5), pp. 593-607. ISSN 1746-0751

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Official URL: https://doi.org/10.2217/rme.14.17

Abstract

Aim: To enumerate and characterize multipotential stromal cells (MSCs) in a cellular bone allograft and compare with fresh age-matched iliac crest bone and bone marrow (BM) aspirate.

Materials and methods: MSC characterization used functional assays, confocal/scanning electron microscopy and whole-genome microarrays. Resident MSCs were enumerated by flow cytometry following enzymatic extraction.

Results: Allograft material contained live osteocytes and proliferative bone-lining cells defined as MSCs by phenotypic and functional capacities. Without cultivation/expansion, the allograft displayed an 'osteoinductive' molecular signature and the presence of CD45-CD271+CD73+CD90+CD105+ MSCs; with a purity over 100-fold that of iliac crest bone. In comparison with BM, MSC numbers enzymatically released from 1 g of cellular allograft were equivalent to approximately 45 ml of BM aspirate.

Conclusion: Cellular allograft bone represents a unique nonimmune material rich in MSCs and osteocytes. This osteoinductive graft represents an attractive alternative to autograft bone or composite/synthetic grafts in orthopedics and broader regenerative medicine settings.

Item Type:	Journal article
Publication Title:	Regenerative Medicine
Creators:	Baboolal, T.G., Boxall, S.A., El-Sherbiny, Y.M., Moseley, T.A., Cuthbert, R.J., Giannoudis, P.V., McGonagle, D. and Jones, E.
Publisher:	Future Medicine Ltd
Date:	2014
Volume:	9
Number:	5
ISSN:	1746-0751
Identifiers:	Number Type 10.2217/rme.14.17 DOI 1199877 Other
Rights:	This article is distributed under the terms of the Creative Commons Attribution License 3.0 which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. To view a copy of the license, visit http://creativecommons.org/licenses/by/3.0/.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	14 Oct 2019 10:46
Last Modified:	14 Oct 2019 14:39
URI:	https://irep.ntu.ac.uk/id/eprint/37950