A platform for screening abiotic/biotic interactions using indicator displacement assays

Michaelis, M ORCID: https://orcid.org/0000-0002-8968-5848, Fayyaz, A, Parambath, M, Köppen, S, Colombi Ciacchi, L, Hanley, QS ORCID: https://orcid.org/0000-0002-8189-9550 and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2019. A platform for screening abiotic/biotic interactions using indicator displacement assays. Langmuir. ISSN 0743-7463

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Abstract

This paper describes novel adaptations of optically sectioned planar format assays to screen compounds for their affinities to materials surfaces. The novel platform, which we name Optical sectioned Indicator Displacement Assays (O-IDA), makes use of displaceable dyes in a format adaptable to high-throughput multi-well plate technologies. We describe two approaches; in the first the dye exhibits fluorescence in both the surface-bound and unbound state. In the second, fluorescence is lost upon displacement of the dye from the surface. Half maximal inhibitory concentration (IC50), binding affinity (Ki), and binding free energy (ΔGads) values can be extracted from the raw data. Representative biomolecules were tested for interactions with silica in aqueous environment and ZnO (0001)-Zn and (10-10) facets in a non-aqueous environment. We provide the first experimental values for both the binding of small molecules to silica and the facet-dependent ZnO binding affinity of key amino acids associated with ZnO-specific oligopeptides. The specific data will be invaluable to those studying interactions at interfaces both experimentally and computationally. O-IDA provides a general framework for the high-throughput screening of molecules binding to materials surfaces, which has important applications in drug delivery, (bio-) catalysis, biosensing and biomaterials engineering.

Item Type:	Journal article
Publication Title:	Langmuir
Creators:	Michaelis, M., Fayyaz, A., Parambath, M., Köppen, S., Colombi Ciacchi, L., Hanley, Q.S. and Perry, C.C.
Publisher:	American Chemical Society
Date:	14 October 2019
ISSN:	0743-7463
Identifiers:	Number Type 10.1021/acs.langmuir.9b03085 DOI 1204147 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	17 Oct 2019 15:32
Last Modified:	31 May 2021 15:15
URI:	https://irep.ntu.ac.uk/id/eprint/38000

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