A light‐activated acyl carrier protein 'trap' for intermediate capture in type II iterative polyketide biocatalysis

Tosin, M, Kilgour, SL, Kilgour, D ORCID: https://orcid.org/0000-0002-3860-7532, Prasongpholchai, P and O'Connor, P, 2019. A light‐activated acyl carrier protein 'trap' for intermediate capture in type II iterative polyketide biocatalysis. Chemistry – A European Journal. ISSN 0947-6539

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Abstract

A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilized for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b by the type II actinorhodin ‘minimal’ polyketide synthase (PKS), a range of putative ACP-bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT-ICR-MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.

Item Type:	Journal article
Publication Title:	Chemistry – A European Journal
Creators:	Tosin, M., Kilgour, S.L., Kilgour, D., Prasongpholchai, P. and O'Connor, P.
Publisher:	Wiley
Date:	9 October 2019
ISSN:	0947-6539
Identifiers:	Number Type 10.1002/chem.201903662 DOI 1202716 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	22 Oct 2019 10:27
Last Modified:	31 May 2021 15:15
URI:	https://irep.ntu.ac.uk/id/eprint/38019

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