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Tosin, M., Kilgour, S.L., Kilgour, D. 
ORCID: 0000-0002-3860-7532, Prasongpholchai, P. and O'Connor, P.,
2019.
A light‐activated acyl carrier protein 'trap' for intermediate capture in type II iterative polyketide biocatalysis.
Chemistry – A European Journal.
ISSN 0947-6539
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Abstract
A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilized for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b by the type II actinorhodin ‘minimal’ polyketide synthase (PKS), a range of putative ACP-bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT-ICR-MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.
| Item Type: | Journal article | ||||||
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| Publication Title: | Chemistry – A European Journal | ||||||
| Creators: | Tosin, M., Kilgour, S.L., Kilgour, D., Prasongpholchai, P. and O'Connor, P. | ||||||
| Publisher: | Wiley | ||||||
| Date: | 9 October 2019 | ||||||
| ISSN: | 0947-6539 | ||||||
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| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 22 Oct 2019 10:27 | ||||||
| Last Modified: | 31 May 2021 15:15 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/38019 |
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