Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

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Maguire, O, Cossarizza, A, Chang, H, Radbruch, A, Acs, A, Adam, D, Adam‐Klages, S, Agace, W, Aghaeepour, N, Akdis, M, Allez, M, Almeida, L, Alvisi, G, Anderson, G, Andrä, I, Annunziato, F, Anselmo, A, Bacher, P, Baldari, C, Bari, S, Barnaba, V, Barros‐Martins, J, Battistini, L, Bauer, W, Baumgart, S, Baumgarth, N, Baumjohann, D, Baying, B, Bebawy, M, Becher, B, Beisker, W, Benes, V, Beyaert, R, Blanco, A, Boardman, D, Bogdan, C, Borger, J, Borsellino, G, Boulais, P, Bradford, J, Brenner, D, Brinkman, R, Brooks, A, Busch, D, Büscher, M, Bushnell, T, Calzetti, F, Cameron, G, Cammarata, I, Cao, X, Cardell, S, Casola, S, Cassatella, M, Cavani, A, Celada, A, Chatenoud, L, Chattopadhyay, P, Chow, S, Christakou, E, Čičin‐Šain, L, Clerici, M, Colombo, F, Cook, L, Cooke, A, Cooper, A, Corbett, A, Cosma, A, Cosmi, L, Coulie, P, Cumano, A, Cvetkovic, L, Dang, V, Dang‐Heine, C, Davey, M, Davies, D, De Biasi, S, Del Zotto, G, Dela Cruz, G, Delacher, M, Della Bella, S, Dellabona, P, Deniz, G, Dessing, M, Di Santo, J, Diefenbach, A, Dieli, F, Dolf, A, Dörner, T, Dress, R, Dudziak, D, Dustin, M, Dutertre, C, Ebner, F, Eckle, S, Edinger, M, Eede, P, Ehrhardt, G, Eich, M, Engel, P, Engelhardt, B, Erdei, A, Esser, C, Everts, B, Evrard, M, Falk, C, Fehniger, T, Felipo‐Benavent, M, Ferry, H, Feuerer, M, Filby, A, Filkor, K, Fillatreau, S, Follo, M, Förster, I, Foster, J, Foulds, G, Frehse, B, Frenette, P, Frischbutter, S, Fritzsche, W, Galbraith, D, Gangaev, A, Garbi, N, Gaudilliere, B, Gazzinelli, R, Geginat, J, Gerner, W, Gherardin, N, Ghoreschi, K, Gibellini, L, Ginhoux, F, Goda, K, Godfrey, D, Goettlinger, C, González‐Navajas, J, Goodyear, C, Gori, A, Grogan, J, Grummitt, D, Grützkau, A, Haftmann, C, Hahn, J, Hammad, H, Hämmerling, G, Hansmann, L, Hansson, G, Harpur, C, Hartmann, S, Hauser, A, Hauser, A, Haviland, D, Hedley, D, Hernández, D, Herrera, G, Herrmann, M, Hess, C, Höfer, T, Hoffmann, P, Hogquist, K, Holland, T, Höllt, T, Holmdahl, R, Hombrink, P, Houston, J, Hoyer, B, Huang, B, Huang, F, Huber, J, Huehn, J, Hundemer, M, Hunter, C, Hwang, W, Iannone, A, Ingelfinger, F, Ivison, S, Jäck, H, Jani, P, Jávega, B, Jonjic, S, Kaiser, T, Kalina, T, Kamradt, T, Kaufmann, S, Keller, B, Ketelaars, S, Khalilnezhad, A, Khan, S, Kisielow, J, Klenerman, P, Knopf, J, Koay, H, Kobow, K, Kolls, J, Kong, W, Kopf, M, Korn, T, Kriegsmann, K, Kristyanto, H, Kroneis, T, Krueger, A, Kühne, J, Kukat, C, Kunkel, D, Kunze‐Schumacher, H, Kurosaki, T, Kurts, C, Kvistborg, P, Kwok, I, Landry, J, Lantz, O, Lanuti, P, LaRosa, F, Lehuen, A, LeibundGut‐Landmann, S, Leipold, M, Leung, L, Levings, M, Lino, A, Liotta, F, Litwin, V, Liu, Y, Ljunggren, H, Lohoff, M, Lombardi, G, Lopez, L, López‐Botet, M, Lovett‐Racke, A, Lubberts, E, Luche, H, Ludewig, B, Lugli, E, Lunemann, S, Maecker, H, Maggi, L, Mair, F, Mair, K, Mantovani, A, Manz, R, Marshall, A, Martínez‐Romero, A, Martrus, G, Marventano, I, Maslinski, W, Matarese, G, Mattioli, A, Maueröder, C, Mazzoni, A, McCluskey, J, McGrath, M, McGuire, H, McInnes, I, Mei, H, Melchers, F, Melzer, S, Mielenz, D, Miller, S, Mills, K, Minderman, H, Mjösberg, J, Moore, J, Moran, B, Moretta, L, Mosmann, T, Müller, S, Multhoff, G, Muñoz, L, Münz, C, Nakayama, T, Nasi, M, Neumann, K, Ng, L, Niedobitek, A, Nourshargh, S, Núñez, G, O'Connor, J, Ochel, A, Oja, A, Ordonez, D, Orfao, A, Orlowski‐Oliver, E, Ouyang, W, Oxenius, A, Palankar, R, Panse, I, Pattanapanyasat, K, Paulsen, M, Pavlinic, D, Penter, L, Peterson, P, Peth, C, Petriz, J, Piancone, F, Pickl, W, Piconese, S, Pinti, M, Pockley, AG ORCID: https://orcid.org/0000-0001-9593-6431, Podolska, M, Poon, Z, Pracht, K, Prinz, I, Pucillo, C, Quataert, S, Quatrini, L, Quinn, K, Radbruch, H, Radstake, T, Rahmig, S, Rahn, H, Rajwa, B, Ravichandran, G, Raz, Y, Rebhahn, J, Recktenwald, D, Reimer, D, Reis e Sousa, C, Remmerswaal, E, Richter, L, Rico, L, Riddell, A, Rieger, A, Robinson, J, Romagnani, C, Rubartelli, A, Ruland, J, Saalmüller, A, Saeys, Y, Saito, T, Sakaguchi, S, Sala‐de‐Oyanguren, F, Samstag, Y, Sanderson, S, Sandrock, I, Santoni, A, Sanz, R, Saresella, M, Sautes‐Fridman, C, Sawitzki, B, Schadt, L, Scheffold, A, Scherer, H, Schiemann, M, Schildberg, F, Schimisky, E, Schlitzer, A, Schlosser, J, Schmid, S, Schmitt, S, Schober, K, Schraivogel, D, Schuh, W, Schüler, T, Schulte, R, Schulz, A, Schulz, S, Scottá, C, Scott‐Algara, D, Sester, D, Shankey, T, Silva‐Santos, B, Simon, A, Sitnik, K, Sozzani, S, Speiser, D, Spidlen, J, Stahlberg, A, Stall, A, Stanley, N, Stark, R, Stehle, C, Steinmetz, T, Stockinger, H, Takahama, Y, Takeda, K, Tan, L, Tárnok, A, Tiegs, G, Toldi, G, Tornack, J, Traggiai, E, Trebak, M, Tree, T, Trotter, J, Trowsdale, J, Tsoumakidou, M, Ulrich, H, Urbanczyk, S, Veen, W, Broek, M, Pol, E, Van Gassen, S, Van Isterdael, G, Lier, R, Veldhoen, M, Vento‐Asturias, S, Vieira, P, Voehringer, D, Volk, H, Borstel, A, Volkmann, K, Waisman, A, Walker, R, Wallace, P, Wang, S, Wang, X, Ward, M, Ward‐Hartstonge, K, Warnatz, K, Warnes, G, Warth, S, Waskow, C, Watson, J, Watzl, C, Wegener, L, Weisenburger, T, Wiedemann, A, Wienands, J, Wilharm, A, Wilkinson, R, Willimsky, G, Wing, J, Winkelmann, R, Winkler, T, Wirz, O, Wong, A, Wurst, P, Yang, J, Yang, J, Yazdanbakhsh, M, Yu, L, Yue, A, Zhang, H, Zhao, Y, Ziegler, S, Zielinski, C, Zimmermann, J and Zychlinsky, A, 2019. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49 (10), pp. 1457-1973. ISSN 0014-2980

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Official URL: https://doi.org/10.1002/eji.201970107

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Item Type:	Journal article
Publication Title:	European Journal of Immunology
Creators:	Maguire, O., Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W., Aghaeepour, N., Akdis, M., Allez, M., Almeida, L., Alvisi, G., Anderson, G., Andrä, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, C., Bari, S., Barnaba, V., Barros‐Martins, J., Battistini, L., Bauer, W., Baumgart, S., Baumgarth, N., Baumjohann, D., Baying, B., Bebawy, M., Becher, B., Beisker, W., Benes, V., Beyaert, R., Blanco, A., Boardman, D., Bogdan, C., Borger, J., Borsellino, G., Boulais, P., Bradford, J., Brenner, D., Brinkman, R., Brooks, A., Busch, D., Büscher, M., Bushnell, T., Calzetti, F., Cameron, G., Cammarata, I., Cao, X., Cardell, S., Casola, S., Cassatella, M., Cavani, A., Celada, A., Chatenoud, L., Chattopadhyay, P., Chow, S., Christakou, E., Čičin‐Šain, L., Clerici, M., Colombo, F., Cook, L., Cooke, A., Cooper, A., Corbett, A., Cosma, A., Cosmi, L., Coulie, P., Cumano, A., Cvetkovic, L., Dang, V., Dang‐Heine, C., Davey, M., Davies, D., De Biasi, S., Del Zotto, G., Dela Cruz, G., Delacher, M., Della Bella, S., Dellabona, P., Deniz, G., Dessing, M., Di Santo, J., Diefenbach, A., Dieli, F., Dolf, A., Dörner, T., Dress, R., Dudziak, D., Dustin, M., Dutertre, C., Ebner, F., Eckle, S., Edinger, M., Eede, P., Ehrhardt, G., Eich, M., Engel, P., Engelhardt, B., Erdei, A., Esser, C., Everts, B., Evrard, M., Falk, C., Fehniger, T., Felipo‐Benavent, M., Ferry, H., Feuerer, M., Filby, A., Filkor, K., Fillatreau, S., Follo, M., Förster, I., Foster, J., Foulds, G., Frehse, B., Frenette, P., Frischbutter, S., Fritzsche, W., Galbraith, D., Gangaev, A., Garbi, N., Gaudilliere, B., Gazzinelli, R., Geginat, J., Gerner, W., Gherardin, N., Ghoreschi, K., Gibellini, L., Ginhoux, F., Goda, K., Godfrey, D., Goettlinger, C., González‐Navajas, J., Goodyear, C., Gori, A., Grogan, J., Grummitt, D., Grützkau, A., Haftmann, C., Hahn, J., Hammad, H., Hämmerling, G., Hansmann, L., Hansson, G., Harpur, C., Hartmann, S., Hauser, A., Hauser, A., Haviland, D., Hedley, D., Hernández, D., Herrera, G., Herrmann, M., Hess, C., Höfer, T., Hoffmann, P., Hogquist, K., Holland, T., Höllt, T., Holmdahl, R., Hombrink, P., Houston, J., Hoyer, B., Huang, B., Huang, F., Huber, J., Huehn, J., Hundemer, M., Hunter, C., Hwang, W., Iannone, A., Ingelfinger, F., Ivison, S., Jäck, H., Jani, P., Jávega, B., Jonjic, S., Kaiser, T., Kalina, T., Kamradt, T., Kaufmann, S., Keller, B., Ketelaars, S., Khalilnezhad, A., Khan, S., Kisielow, J., Klenerman, P., Knopf, J., Koay, H., Kobow, K., Kolls, J., Kong, W., Kopf, M., Korn, T., Kriegsmann, K., Kristyanto, H., Kroneis, T., Krueger, A., Kühne, J., Kukat, C., Kunkel, D., Kunze‐Schumacher, H., Kurosaki, T., Kurts, C., Kvistborg, P., Kwok, I., Landry, J., Lantz, O., Lanuti, P., LaRosa, F., Lehuen, A., LeibundGut‐Landmann, S., Leipold, M., Leung, L., Levings, M., Lino, A., Liotta, F., Litwin, V., Liu, Y., Ljunggren, H., Lohoff, M., Lombardi, G., Lopez, L., López‐Botet, M., Lovett‐Racke, A., Lubberts, E., Luche, H., Ludewig, B., Lugli, E., Lunemann, S., Maecker, H., Maggi, L., Mair, F., Mair, K., Mantovani, A., Manz, R., Marshall, A., Martínez‐Romero, A., Martrus, G., Marventano, I., Maslinski, W., Matarese, G., Mattioli, A., Maueröder, C., Mazzoni, A., McCluskey, J., McGrath, M., McGuire, H., McInnes, I., Mei, H., Melchers, F., Melzer, S., Mielenz, D., Miller, S., Mills, K., Minderman, H., Mjösberg, J., Moore, J., Moran, B., Moretta, L., Mosmann, T., Müller, S., Multhoff, G., Muñoz, L., Münz, C., Nakayama, T., Nasi, M., Neumann, K., Ng, L., Niedobitek, A., Nourshargh, S., Núñez, G., O'Connor, J., Ochel, A., Oja, A., Ordonez, D., Orfao, A., Orlowski‐Oliver, E., Ouyang, W., Oxenius, A., Palankar, R., Panse, I., Pattanapanyasat, K., Paulsen, M., Pavlinic, D., Penter, L., Peterson, P., Peth, C., Petriz, J., Piancone, F., Pickl, W., Piconese, S., Pinti, M., Pockley, A.G., Podolska, M., Poon, Z., Pracht, K., Prinz, I., Pucillo, C., Quataert, S., Quatrini, L., Quinn, K., Radbruch, H., Radstake, T., Rahmig, S., Rahn, H., Rajwa, B., Ravichandran, G., Raz, Y., Rebhahn, J., Recktenwald, D., Reimer, D., Reis e Sousa, C., Remmerswaal, E., Richter, L., Rico, L., Riddell, A., Rieger, A., Robinson, J., Romagnani, C., Rubartelli, A., Ruland, J., Saalmüller, A., Saeys, Y., Saito, T., Sakaguchi, S., Sala‐de‐Oyanguren, F., Samstag, Y., Sanderson, S., Sandrock, I., Santoni, A., Sanz, R., Saresella, M., Sautes‐Fridman, C., Sawitzki, B., Schadt, L., Scheffold, A., Scherer, H., Schiemann, M., Schildberg, F., Schimisky, E., Schlitzer, A., Schlosser, J., Schmid, S., Schmitt, S., Schober, K., Schraivogel, D., Schuh, W., Schüler, T., Schulte, R., Schulz, A., Schulz, S., Scottá, C., Scott‐Algara, D., Sester, D., Shankey, T., Silva‐Santos, B., Simon, A., Sitnik, K., Sozzani, S., Speiser, D., Spidlen, J., Stahlberg, A., Stall, A., Stanley, N., Stark, R., Stehle, C., Steinmetz, T., Stockinger, H., Takahama, Y., Takeda, K., Tan, L., Tárnok, A., Tiegs, G., Toldi, G., Tornack, J., Traggiai, E., Trebak, M., Tree, T., Trotter, J., Trowsdale, J., Tsoumakidou, M., Ulrich, H., Urbanczyk, S., Veen, W., Broek, M., Pol, E., Van Gassen, S., Van Isterdael, G., Lier, R., Veldhoen, M., Vento‐Asturias, S., Vieira, P., Voehringer, D., Volk, H., Borstel, A., Volkmann, K., Waisman, A., Walker, R., Wallace, P., Wang, S., Wang, X., Ward, M., Ward‐Hartstonge, K., Warnatz, K., Warnes, G., Warth, S., Waskow, C., Watson, J., Watzl, C., Wegener, L., Weisenburger, T., Wiedemann, A., Wienands, J., Wilharm, A., Wilkinson, R., Willimsky, G., Wing, J., Winkelmann, R., Winkler, T., Wirz, O., Wong, A., Wurst, P., Yang, J., Yang, J., Yazdanbakhsh, M., Yu, L., Yue, A., Zhang, H., Zhao, Y., Ziegler, S., Zielinski, C., Zimmermann, J. and Zychlinsky, A.
Publisher:	Wiley
Date:	October 2019
Volume:	49
Number:	10
ISSN:	0014-2980
Identifiers:	Number Type 10.1002/eji.201970107 DOI 1216383 Other
Rights:	© 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.
Divisions:	Schools > School of Science and Technology
Record created by:	Jill Tomkinson
Date Added:	11 Dec 2019 13:59
Last Modified:	11 Dec 2019 13:59
URI:	https://irep.ntu.ac.uk/id/eprint/38850