Treatment of human lens epithelium with high levels of nanoceria leads to reactive oxygen species mediated apoptosis

Cave, G ORCID: https://orcid.org/0000-0002-4167-1332, Barnett, Y, Pierscionek, B and Hanafy, B, 2020. Treatment of human lens epithelium with high levels of nanoceria leads to reactive oxygen species mediated apoptosis. Molecules, 25 (3): 441. ISSN 1420-3049

Preview

Text 1276467_Cave.pdf - Published version Download (4MB) | Preview

Abstract

Nanoceria (cerium oxide nanoparticles) have been shown to protect human lens epithelial cells (HLECs) from oxidative stress when used at low concentrations. However, there is a lack of understanding about the mechanism of the cytotoxic and genotoxic effects of nanoceria when used at higher concentrations. Here, we investigated the impact of 24-hour exposure to nanoceria in HLECs. Nanoceria’s effects on basal reactive oxygen species (ROS), mitochondrial morphology, membrane potential, ATP, genotoxicity, caspase activation and apoptotic hallmarks were investigated. Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) studies on isolated mitochondria revealed significant uptake and localization of nanoceria in the mitochondria. At high nanoceria concentrations (400 µg mL−1), intracellular levels of ROS were increased and the HLECs exhibited classical hallmarks of apoptosis. These findings concur with the cells maintaining normal ATP levels necessary to execute the apoptotic process. These results highlight the need for nanoceria dose-effect studies on a range of cells and tissues to identify therapeutic concentrations in vitro or in vivo

Item Type:	Journal article
Publication Title:	Molecules
Creators:	Cave, G., Barnett, Y., Pierscionek, B. and Hanafy, B.
Publisher:	MDPI
Date:	21 January 2020
Volume:	25
Number:	3
ISSN:	1420-3049
Identifiers:	Number Type 10.3390/molecules25030441 DOI 1276467 Other
Rights:	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	23 Jan 2020 13:54
Last Modified:	23 Jan 2020 13:54
URI:	https://irep.ntu.ac.uk/id/eprint/39061

Actions (login required)

Edit View

Statistics

Views

Downloads