The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence

Wrobel, A, Saragliadis, A, Pérez‐Ortega, J, Sittman, C, Göttig, S, Liskiewicz, K, Spence, MH, Schneider, K, Leo, JC ORCID: https://orcid.org/0000-0002-7066-7527, Arenas, J and Linke, D, 2020. The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence. Environmental Microbiology, 22 (7), pp. 2939-2955. ISSN 1462-2912

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Abstract

Yersinia ruckeri causes enteric redmouth disease (ERM) that mainly affects salmonid fishes and leads to significant economic losses in the aquaculture industry. An increasing number of outbreaks and the lack of effective vaccines against some serotypes necessitates novel measures to control ERM. Importantly, Y. ruckeri survives in the environment for long periods, presumably by forming biofilms. How the pathogen forms biofilms and which molecular factors are involved in this process, remains unclear. Yersinia ruckeri produces two surface‐exposed adhesins, belonging to the inverse autotransporters (IATs), called Y. ruckeri invasin (YrInv) and Y. ruckeri invasin‐like molecule (YrIlm). Here, we investigated whether YrInv and YrIlm play a role in biofilm formation and virulence. Functional assays revealed that YrInv and YrIlm promote biofilm formation on different abiotic substrates. Confocal microscopy revealed that they are involved in microcolony interaction and formation, respectively. The effect of both IATs on biofilm formation correlated with the presence of different biopolymers in the biofilm matrix, including extracellular DNA, RNA and proteins. Moreover, YrInv and YrIlm contributed to virulence in the Galleria mellonella infection model. Taken together, we propose that both IATs are possible targets for the development of novel diagnostic and preventative strategies to control ERM.

Item Type:	Journal article
Publication Title:	Environmental Microbiology
Creators:	Wrobel, A., Saragliadis, A., Pérez‐Ortega, J., Sittman, C., Göttig, S., Liskiewicz, K., Spence, M.H., Schneider, K., Leo, J.C., Arenas, J. and Linke, D.
Publisher:	Wiley-Blackwell
Date:	July 2020
Volume:	22
Number:	7
ISSN:	1462-2912
Identifiers:	Number Type 10.1111/1462-2920.15051 DOI 1326742 Other
Rights:	© 2020 the authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Divisions:	Schools > School of Science and Technology
Record created by:	Jill Tomkinson
Date Added:	19 May 2020 15:24
Last Modified:	14 Jul 2020 08:02
URI:	https://irep.ntu.ac.uk/id/eprint/39875

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