Succession of Bifidobacterium longum strains in response to a changing early life nutritional environment reveals dietary substrate adaptations

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Kujawska, M, La Rosa, SL, Roger, LC, Pope, PB, Hoyles, L ORCID: https://orcid.org/0000-0002-6418-342X, McCartney, AL and Hall, LJ, 2020. Succession of Bifidobacterium longum strains in response to a changing early life nutritional environment reveals dietary substrate adaptations. iScience. ISSN 2589-0042

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Official URL: https://doi.org/10.1016/j.isci.2020.101368

Abstract

Diet-microbe interactions play a crucial role in modulation of the early life microbiota and infant health. Bifidobacterium dominates the breast-fed infant gut and may persist in individuals during transition from a milk-based to a more diversified diet. Here, we investigated adaptation of B. longum to the changing nutritional environment. Genomic characterisation of 75 strains isolated from nine either exclusively breast- or formula-fed (pre-weaning) infants in their first 18 months revealed subspecies- and strain-specific intra-individual genomic diversity with respect to carbohydrate metabolism, which corresponded to different dietary stages. Complementary phenotypic studies indicated strain-specific differences in utilisation of human milk oligosaccharides and plant carbohydrates, while proteomic profiling identified gene clusters involved in metabolism of selected carbohydrates. Our results indicate a strong link between infant diet and B. longum diversity and provide additional insights into possible competitive advantage mechanisms of this Bifidobacterium species and its persistence in a single host.

Item Type:	Journal article
Publication Title:	iScience
Creators:	Kujawska, M., La Rosa, S.L., Roger, L.C., Pope, P.B., Hoyles, L., McCartney, A.L. and Hall, L.J.
Publisher:	Cell Press
Date:	14 July 2020
ISSN:	2589-0042
Identifiers:	Number Type 10.1016/j.isci.2020.101368 DOI S2589-0042(20)30556-3 Publisher Item Identifier 1345538 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jill Tomkinson
Date Added:	22 Jul 2020 13:42
Last Modified:	31 May 2021 15:18
URI:	https://irep.ntu.ac.uk/id/eprint/40261