Leukocyte function in myelopoliferative disorders

El-Maallem, H, 1976. Leukocyte function in myelopoliferative disorders. PhD, Nottingham Trent University.

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Abstract

Myeloproliferative disorders are characterised by abnormal proliferation of the myeloid tissues in the bone marrow and in metaplastic sites including the liver and spleen. When this work started in 1973, very little was known about the functional and enzymic abnormalities of the leukocytes in these disorders. During the last three years I have been able to set up quantitative and qualitative procedures for the estimation of leukocytes primary granular enzymes, such as peroxidase, acid phosphatase and 3-glucuronidase as well as secondary granular enzymes, such as alkaline phosphatase. Also, I have been able to set up a quantitative method for the estimation of the neutrophil function which included phagocytosis and killing of Candida guillermondii as well as degranulation and H2O2 production during phagocytosis.

Fifty healthy subjects, 21 males and 29 females, ranging between 17 and 74 years of age, were investigated. These were compared to 13 Chronic Granulocytic Leukaemia, 12 Myeloid Metaplasia and 7 Polycythaemia Rubra Vera. The principle abnormalities that have been demonstrated in the Chronic Granulocytic Leukaemia neutrophils were the significant reduction in the fungicidal capacity, the impairment of degranulation and the significant reduction in H2O2 production during phagocytosis. Myeloid metaplasia neutrophils had a significant reduction in the fungicidal capacity and a low peroxidase level which correlated significantly with the corresponding fungicidal ability. Polycythaemia Rubra Vera neutrophils, also, had a significant reduction in the fungicidal capacity.

Quantitative and comparative studies on the cytochemistry and function of the leukocytes from these myeloproliferative disorders was valuable in distinguishing the different disorders and throwing some light on the different mechanisms which appear to be responsible for their functional impairment.

Item Type:	Thesis
Creators:	El-Maallem, H.
Date:	1976
ISBN:	9781369324846
Identifiers:	Number Type PQ10290235 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	24 Jun 2021 10:47
Last Modified:	18 Oct 2023 15:49
URI:	https://irep.ntu.ac.uk/id/eprint/43202

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