The in vitro uptake of catecholamines into rat brain preparations

Brammer, NT, 1985. The in vitro uptake of catecholamines into rat brain preparations. PhD, Nottingham Trent University.

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Abstract

The uptake of the catecholamines, dopamine and noradrenaline, was investigated in a number of rat brain preparations. Kinetic analysis of the uptake of dopamine in striatal slices indicated that at least two distinct uptake systems for the compound existed in this region; one with high affinity for dopamine, the other possessing low affinity for dopamine. Distinct high and low affinity uptake systems were also observed for the uptake of dopamine into slices of cerebellum, and the uptake of noradrenaline into both striatal slices and slices of cerebellum.

The uptake of dopamine into striatal slices was further investigated using a number of compounds to inhibit the uptake process. The rank order of decreasing potency of the compounds was shown to be different for the high and low affinity uptake systems in this region.

The uptake of dopamine was also studied in isolated striatal cells, striatal homogenates and striatal synaptosomes. Two uptake systems with differing kinetic parameters were observed in isolated striatal cells, but only a high affinity system was detected in striatal synaptosomes. The results of the experiments using striatal homogenates were inconclusive.

The metabolism of dopamine and noradrenaline in striatal slices following incubation in a high concentration of catecholamine was investigated.

The major metabolites of dopamine' and noradrenaline were shown to be 3,4-dihydroxyphenylacetic acid and 3,4-dihydroxyphenylethylglycol, respectively. The accumulation of these metabolites following incubation in their respective precursors at 100 μM was not significantly affected by pretreatment of the rats with 6-hydroxydopamine.

Item Type:	Thesis
Creators:	Brammer, N.T.
Date:	1985
ISBN:	9781369325829
Identifiers:	Number Type PQ10290333 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	08 Jul 2021 10:08
Last Modified:	22 May 2024 15:16
URI:	https://irep.ntu.ac.uk/id/eprint/43381

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