Bioactivity and antibacterial behaviors of nanostructured lithium-doped hydroxyapatite for bone scaffold application

Keikhosravani, P, Maleki-Ghaleh, H, Kahaie Khosrowshahi, A, Bodaghi, M ORCID: https://orcid.org/0000-0002-0707-944X, Dargahi, Z, Kavanlouei, M, Khademi-Azandehi, P, Fallah, A, Beygi-Khosrowshahi, Y and Siadati, MH, 2021. Bioactivity and antibacterial behaviors of nanostructured lithium-doped hydroxyapatite for bone scaffold application. International Journal of Molecular Sciences, 22 (17): 9214. ISSN 1661-6596

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Abstract

The material for bone scaffold replacement should be biocompatible and antibacterial to prevent scaffold-associated infection. We biofunctionalized the hydroxyapatite (HA) properties by doping it with lithium (Li). The HA and 4 Li-doped HA (0.5, 1.0, 2.0, 4.0 wt.%) samples were investigated to find the most suitable Li content for both aspects. The synthesized nanoparticles, by the mechanical alloying method, were cold-pressed uniaxially and then sintered for 2 h at 1250 °C. Characterization using field-emission scanning electron microscopy (FE-SEM) revealed particle sizes in the range of 60 to 120 nm. The XRD analysis proved the formation of HA and Li-doped HA nanoparticles with crystal sizes ranging from 59 to 89 nm. The bioactivity of samples was investigated in simulated body fluid (SBF), and the growth of apatite formed on surfaces was evaluated using SEM and EDS. Cellular behavior was estimated by MG63 osteoblast-like cells. The results of apatite growth and cell analysis showed that 1.0 wt.% Li doping was optimal to maximize the bioactivity of HA. Antibacterial characteristics against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were performed by colony-forming unit (CFU) tests. The results showed that Li in the structure of HA increases its antibacterial properties. HA biofunctionalized by Li doping can be considered a suitable option for the fabrication of bone scaffolds due to its antibacterial and unique bioactivity properties.

Item Type:	Journal article
Publication Title:	International Journal of Molecular Sciences
Creators:	Keikhosravani, P., Maleki-Ghaleh, H., Kahaie Khosrowshahi, A., Bodaghi, M., Dargahi, Z., Kavanlouei, M., Khademi-Azandehi, P., Fallah, A., Beygi-Khosrowshahi, Y. and Siadati, M.H.
Publisher:	MDPI AG
Date:	26 August 2021
Volume:	22
Number:	17
ISSN:	1661-6596
Identifiers:	Number Type 10.3390/ijms22179214 DOI 1469742 Other
Rights:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	08 Sep 2021 14:58
Last Modified:	08 Sep 2021 14:58
URI:	https://irep.ntu.ac.uk/id/eprint/44144

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