Microfluidic integrated capacitive biosensor for C-reactive protein label-free and real-time detection

Liu, D, Zhou, L, Huang, L, Zuo, Z, Ho, V, Jin, L, Lu, Y, Chen, X ORCID: https://orcid.org/0000-0001-7091-1414, Zhao, J, Qian, D, Liu, H and Mao, H, 2021. Microfluidic integrated capacitive biosensor for C-reactive protein label-free and real-time detection. Analyst, 146 (17), pp. 5380-5388. ISSN 0003-2654

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Abstract

A microfluidic chip has been integrated with a capacitive biosensor based on mass-producible threedimensional (3D) interdigital electrode arrays. To achieve the monitoring of biosensor preparation and cardiac- and periodontitis-related biomarkers, all the processes were detected in a continuously on-site way. Fabrication steps for the microfluidic chip-bonded 3D interdigital capacitor biosensor include gold thiol modification, the activation of EDC/sulfo-NHS, and the bioconjugation of antibodies. Fluorescent characterization and X-ray photoelectron spectroscopy analysis were applied to assess the successful immobilization of the C-reactive protein (CRP) antibody. The experimental results indicate the good specificity and high sensitivity of the microfluidic integrated 3D capacitive biosensor. The limit of detection of the 3D capacitive biosensor for CRP label-free detection was about 1 pg mL−1. This 3D capacitive biosensor with integrated microfluidics is mass-producible and has achieved the on-site continuous detection of cardiac- and periodontitis-related biomarkers with high performance.

Item Type:	Journal article
Publication Title:	Analyst
Creators:	Liu, D., Zhou, L., Huang, L., Zuo, Z., Ho, V., Jin, L., Lu, Y., Chen, X., Zhao, J., Qian, D., Liu, H. and Mao, H.
Publisher:	Royal Society of Chemistry (RSC)
Date:	26 July 2021
Volume:	146
Number:	17
ISSN:	0003-2654
Identifiers:	Number Type 10.1039/d1an00464f DOI 1470892 Other
Rights:	© Royal Society of Chemistry 2021.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	17 Sep 2021 08:37
Last Modified:	17 Sep 2021 08:37
URI:	https://irep.ntu.ac.uk/id/eprint/44199

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