Evaluation of human bone marrow mesenchymal stromal cell (MSC) functions on a biomorphic rattan-wood-derived scaffold: a comparison between cultured and uncultured MSCs

Ganguly, P, El-Jawhari, JJ ORCID: https://orcid.org/0000-0002-0580-4492, Vun, J, Giannoudis, PV and Jones, EA, 2021. Evaluation of human bone marrow mesenchymal stromal cell (MSC) functions on a biomorphic rattan-wood-derived scaffold: a comparison between cultured and uncultured MSCs. Bioengineering, 9 (1): 1. ISSN 2306-5354

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Abstract

The reconstruction of large bone defects requires the use of biocompatible osteoconductive scaffolds. These scaffolds are often loaded with the patient’s own bone marrow (BM) cells to facilitate osteoinductivity and biological potency. Scaffolds that are naturally sourced and fabricated through biomorphic transitions of rattan wood (B-HA scaffolds) offer a unique advantage of higher mechanical strength and bioactivity. In this study, we investigated the ability of a biomorphic B-HA scaffold (B-HA) to support the attachment, survival and gene expression profile of human uncultured BM-derived mesenchymal stromal cells (BMSCs, n = 6) and culture expanded MSCs (cMSCs, n = 7) in comparison to a sintered, porous HA scaffold (S-HA). B-HA scaffolds supported BMSC attachment (average 98%) and their survival up to 4 weeks in culture. Flow cytometry confirmed the phenotype of cMSCs on the scaffolds. Gene expression indicated clear segregation between cMSCs and BMSCs with MSC osteogenesis- and adipogenesis-related genes including RUNX2, PPARγ, ALP and FABP4 being higher expressed in BMSCs. These data indicated a unique transcriptional signature of BMSCs that was distinct from that of cMSCs regardless of the type of scaffold or time in culture. There was no statistical difference in the expression of osteogenic genes in BMSCs or cMSCs in B-HA compared to S-HA. VEGF release from cMSCs co-cultured with human endothelial cells (n = 4) on B-HA scaffolds suggested significantly higher supernatant concentration with endothelial cells on day 14. This indicated a potential mechanism for providing vasculature to the repair area when such scaffolds are used for treating large bone defects.

Item Type:	Journal article
Publication Title:	Bioengineering
Creators:	Ganguly, P., El-Jawhari, J.J., Vun, J., Giannoudis, P.V. and Jones, E.A.
Publisher:	MDPI AG
Date:	21 December 2021
Volume:	9
Number:	1
ISSN:	2306-5354
Identifiers:	Number Type 10.3390/bioengineering9010001 DOI 1505998 Other
Rights:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	07 Jan 2022 10:23
Last Modified:	07 Jan 2022 10:23
URI:	https://irep.ntu.ac.uk/id/eprint/45184

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