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Helal, M.A., Abdel-Gawad, A.M., Kandil, O.M., Khalifa, M.M.E., Morrison, A.A., Bartley, D.J., Cave, G.W.V. 
ORCID: 0000-0002-4167-1332 and Elsheikha, H.M.,
2022.
Microfluidic‐based formulation of essential oils-loaded chitosan coated PLGA particles enhances their bioavailability and nematocidal activity.
Pharmaceutics, 14 (10): 2030.
ISSN 1999-4923
Abstract
In this study, poly(lactic-co-glycolic) acid (PLGA) particles were synthesized and coated with chitosan. Three essential oil (EO) components (linalool, geraniol and eugenol) were entrapped inside these PLGA particles by using continuous flow-focusing microfluidic method and a partially water-miscible solvent mixture [dichloromethane: acetone mixture (1:10)]. Encapsulation of EOs in PLGA particles was confirmed by Fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray diffraction, with encapsulation efficiencies 95.14, 79.68 and 71.34% and loading capacities 8.88, 8.38 and 5.65% in particles entrapped with eugenol, linalool and geraniol, respectively. The EO release from the loaded particles exhibited an initial burst release in the first 8 h followed by a sustained release phase at significantly slower rates from the coated particles, extending beyond 5 days. EOs encapsulated in chitosan coated particles up to 5 μg/mL were not cytotoxic to bovine gut cell line (FFKD-1-R) and had no adverse effect on cell growth and membrane integrity compared with free EOs or uncoated particles. The coated particles loaded with combined EOs significantly inhibited the motility of larval and adult stages of the abomasal nematodes Haemonchus contortus and Trichostrongylus axei at ≥1.25 µg/mL. This nematocidal effect was accompanied by considerable cuticular damage in the treated worms, reflecting a synergistic effect of combined EOs and additive effect of chitosan. These results show that encapsulation of EO components, with a potent anthelmintic activity, in chitosan coated PLGA particles improve the bioavailability and efficacy of EOs against ovine gastrointestinal nematodes.
| Item Type: | Journal article | ||||||
|---|---|---|---|---|---|---|---|
| Publication Title: | Pharmaceutics | ||||||
| Creators: | Helal, M.A., Abdel-Gawad, A.M., Kandil, O.M., Khalifa, M.M.E., Morrison, A.A., Bartley, D.J., Cave, G.W.V. and Elsheikha, H.M. | ||||||
| Publisher: | MDPI | ||||||
| Date: | 23 September 2022 | ||||||
| Volume: | 14 | ||||||
| Number: | 10 | ||||||
| ISSN: | 1999-4923 | ||||||
| Identifiers: |
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| Rights: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Laura Ward | ||||||
| Date Added: | 22 Sep 2022 10:21 | ||||||
| Last Modified: | 15 Nov 2022 15:32 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/47075 |
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