Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii

Fakaea, LB, Harun, MSR, Ting, DSJ, Dua, HS, Cave, GWV ORCID: https://orcid.org/0000-0002-4167-1332, Zhu, X-Q, Stevenson, CW and Elsheikha, HM, 2023. Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii. Acta Tropica, 237: 106729. ISSN 0001-706X

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Abstract

We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (625–5000 µg/mL) inhibited trophozoite replication within 24–72 h. C. sinensis SE exhibited a dose-dependent inhibition of encystation, with a marked cysticidal activity at 2500–5000 µg/mL. Two constituents of C. sinensis, namely epigallocatechin-3-gallate and caffeine, at 100 μM and 200 μM respectively, significantly inhibited both trophozoite replication and encystation. Cytotoxicity analysis showed that 156.25–2500 µg/mL of SE was not toxic to human corneal epithelial cells, while up to 625 µg/mL was not toxic to Madin-Darby canine kidney cells. This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.

Item Type:	Journal article
Publication Title:	Acta Tropica
Creators:	Fakaea, L.B., Harun, M.S.R., Ting, D.S.J., Dua, H.S., Cave, G.W.V., Zhu, X.-Q., Stevenson, C.W. and Elsheikha, H.M.
Publisher:	Elsevier
Date:	January 2023
Volume:	237
ISSN:	0001-706X
Identifiers:	Number Type 10.1016/j.actatropica.2022.106729 DOI S0001706X22004211 Publisher Item Identifier 1612498 Other
Rights:	© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	08 Nov 2022 10:03
Last Modified:	08 Nov 2022 10:03
URI:	https://irep.ntu.ac.uk/id/eprint/47345

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