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Fakaea, L.B., Harun, M.S.R., Ting, D.S.J., Dua, H.S., Cave, G.W.V. 
ORCID: 0000-0002-4167-1332, Zhu, X.-Q., Stevenson, C.W. and Elsheikha, H.M.,
2023.
Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii.
Acta Tropica, 237: 106729.
ISSN 0001-706X
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Abstract
We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (625–5000 µg/mL) inhibited trophozoite replication within 24–72 h. C. sinensis SE exhibited a dose-dependent inhibition of encystation, with a marked cysticidal activity at 2500–5000 µg/mL. Two constituents of C. sinensis, namely epigallocatechin-3-gallate and caffeine, at 100 μM and 200 μM respectively, significantly inhibited both trophozoite replication and encystation. Cytotoxicity analysis showed that 156.25–2500 µg/mL of SE was not toxic to human corneal epithelial cells, while up to 625 µg/mL was not toxic to Madin-Darby canine kidney cells. This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.
| Item Type: | Journal article | ||||||||
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| Publication Title: | Acta Tropica | ||||||||
| Creators: | Fakaea, L.B., Harun, M.S.R., Ting, D.S.J., Dua, H.S., Cave, G.W.V., Zhu, X.-Q., Stevenson, C.W. and Elsheikha, H.M. | ||||||||
| Publisher: | Elsevier | ||||||||
| Date: | January 2023 | ||||||||
| Volume: | 237 | ||||||||
| ISSN: | 0001-706X | ||||||||
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| Rights: | © 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). | ||||||||
| Divisions: | Schools > School of Science and Technology | ||||||||
| Record created by: | Linda Sullivan | ||||||||
| Date Added: | 08 Nov 2022 10:03 | ||||||||
| Last Modified: | 08 Nov 2022 10:03 | ||||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/47345 |
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