Triphasic 3D in vitro model of bone-tendon-muscle interfaces to study their regeneration

Balestri, W ORCID: https://orcid.org/0000-0001-9111-8116, Hickman, GJ ORCID: https://orcid.org/0000-0002-4632-9229, Morris, RH ORCID: https://orcid.org/0000-0001-5511-3457, Hunt, JA ORCID: https://orcid.org/0000-0002-5168-4778 and Reinwald, Y ORCID: https://orcid.org/0000-0001-6733-605X, 2023. Triphasic 3D in vitro model of bone-tendon-muscle interfaces to study their regeneration. Cells, 12 (2): 313. ISSN 2073-4409

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Abstract

The transition areas between different tissues known as tissue interfaces have limited ability to regenerate after damage which can lead to incomplete healing. Previous studies focussed on single interfaces most commonly bone-tendon and bone-cartilage. Here, we develop a 3D in vitro model to study the regeneration of the bone-tendon-muscle interface. The 3D model was prepared from collagen and agarose, with different concentrations of hydroxyapatite to graduate the tissues from bone to muscle, resulting in a stiffness gradient. This graduated structure was fabricated using in-direct 3D printing to provide biologically relevant surface topographies. MG-63, human dermal fi-broblast and Sket.4U cells were found suitable cell models for bone, tendon, and muscle respec-tively. The biphasic and triphasic hydrogels composing the 3D model were shown to be suitable for cell growth. Cells were co-cultured on the 3D model over 21 days before assessing cell prolifera-tion, metabolic activity, viability, cytotoxicity, tissue-specific markers, and matrix deposition to de-termine interface formations. The studies were conducted in a newly developed growth chamber that allowed cell communication while the cell culture media was compartmentalised. The 3D model promoted cell viability, tissue-specific marker expression and new matrix deposition over 21 days showing promise for the development of new interfaces

Item Type:	Journal article
Publication Title:	Cells
Creators:	Balestri, W., Hickman, G.J., Morris, R.H., Hunt, J.A. and Reinwald, Y.
Publisher:	MDPI
Date:	10 January 2023
Volume:	12
Number:	2
ISSN:	2073-4409
Identifiers:	Number Type 10.3390/cells12020313 DOI 1633128 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	13 Jan 2023 11:55
Last Modified:	24 Aug 2023 14:39
URI:	https://irep.ntu.ac.uk/id/eprint/47856

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