Lytic bacteriophage vB_KmiS-Kmi2C disrupts biofilms formed by members of the Klebsiella oxytoca complex, and represents a novel virus family and genus

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Newberry, F ORCID: https://orcid.org/0000-0002-7253-6950, Shibu, P, Smith-Zaitlik, T, Eladawy, M, McCartney, AL, Hoyles, L ORCID: https://orcid.org/0000-0002-6418-342X and Negus, D ORCID: https://orcid.org/0000-0001-9047-4565, 2023. Lytic bacteriophage vB_KmiS-Kmi2C disrupts biofilms formed by members of the Klebsiella oxytoca complex, and represents a novel virus family and genus. Journal of Applied Microbiology, 134 (4): lxad079. ISSN 1364-5072

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Official URL: https://doi.org/10.1093/jambio/lxad079

Abstract

Aims:This study aimed to characterise the lytic phage vB_KmiS-Kmi2C, isolated from sewage water on a GES-positive strain of Klebsiella michiganensis.

Methods and results: Comparative phylogenetic and network-based analyses were used to characterise the genome of phage vB_KmiS-Kmi2C (circular genome of 42,234 bp predicted to encode 55 genes), demonstrating it shared little similarity with other known phages. The phage was lytic on clinical strains of K. oxytoca (n=2) and K. michiganensis (n=4), and was found to both prevent biofilm formation and disrupt established biofilms produced by these strains.

Conclusions: We have identified a phage capable of killing clinically relevant members of the Klebsiella oxytoca complex (KoC). The phage represents a novel virus family (proposed name Dilsviridae) and genus (proposed name Dilsvirus).

Significance and impact of the study: Identification a novel lytic phage active against clinically relevant strains of the KoC provides an alternative to antibiotics to treat these increasingly antimicrobial-resistant opportunistic pathogens. The unusual way in which the phage can disrupt established biofilms may allow us to identify novel phage-based approaches for biofilm remediation in the future.

Item Type:	Journal article
Publication Title:	Journal of Applied Microbiology
Creators:	Newberry, F., Shibu, P., Smith-Zaitlik, T., Eladawy, M., McCartney, A.L., Hoyles, L. and Negus, D.
Publisher:	Wiley
Date:	17 April 2023
Volume:	134
Number:	4
ISSN:	1364-5072
Identifiers:	Number Type 10.1093/jambio/lxad079 DOI 1750034 Other
Rights:	This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Applied Microbiology following peer review. The version of record NEWBERRY, F. , SHIBU, P., SMITH-ZAITLIK, T., ELADAWY, M., MCCARTNEY, A.L., HOYLES, L. and NEGUS, D. , 2023. Lytic bacteriophage vB_KmiS-Kmi2C disrupts biofilms formed by members of the Klebsiella oxytoca complex, and represents a novel virus family and genus. Journal of Applied Microbiology, 134 (4): lxad079 is available online at: https://doi.org/10.1093/jambio/lxad079
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	13 Apr 2023 15:42
Last Modified:	17 Apr 2024 03:00
URI:	https://irep.ntu.ac.uk/id/eprint/48735