Enhanced in vitro and in vivo anticancer activity through the development of sunitinib-loaded nanoniosomes with controlled release and improved uptake

Dehghan, S, Naghipour, A, Zomorodi Anbaji, F, Golshanrad, P, Mirazi, H, Adelnia, H, Bodaghi, M ORCID: https://orcid.org/0000-0002-0707-944X and Farasati Far, B, 2023. Enhanced in vitro and in vivo anticancer activity through the development of sunitinib-loaded nanoniosomes with controlled release and improved uptake. International Journal of Pharmaceutics, 640: 122977. ISSN 0378-5173

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Abstract

This study aims to develop sunitinib niosomal formulations and assess their in-vitro anti-cancer efficiency against lung cancer cell line, A549. Sunitinib, a highly effective anticancer drug, was loaded in the niosome with high encapsulation efficiency. Collagen was coated on the surface of the niosome for enhanced cellular uptake and prolonged circulation time. Different formulations were produced, while response surface methodology was utilized to optimize the formulations. The stability of the formulations was evaluated over a 2-month period, revealing the importance of collagen coating. MTT assay demonstrated dose-dependent cytotoxicity for all formulations against lung cancer cells. Scratch assay test suggested antiproliferative efficacy of the formulations. The flow cytometry data confirmed the improved cytotoxicity with enhanced apoptosis rate when different formulations used. The 2D fluorescent images proved the presence of drug-containing niosomes in the tumor cells. The activation of the apoptotic pathway leading to protein synthesis was confirmed using an ELISA assay, which specifically evaluated the presence of cas3 and cas7. The results of this study indicated the antiproliferative efficacy of optimized niosomal formulations and their mechanism of action. Therefore, niosomes could be utilized as a suitable carrier for delivering sunitinib into lung cancer cells, paving the way for future clinical studies.

Item Type:	Journal article
Publication Title:	International Journal of Pharmaceutics
Creators:	Dehghan, S., Naghipour, A., Zomorodi Anbaji, F., Golshanrad, P., Mirazi, H., Adelnia, H., Bodaghi, M. and Farasati Far, B.
Publisher:	Elsevier BV
Date:	10 June 2023
Volume:	640
ISSN:	0378-5173
Identifiers:	Number Type 10.1016/j.ijpharm.2023.122977 DOI 1759301 Other
Rights:	© 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	12 May 2023 10:00
Last Modified:	12 May 2023 10:00
URI:	https://irep.ntu.ac.uk/id/eprint/48949

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