Modelling skeletal muscle ageing and repair in vitro

Tarum, J ORCID: https://orcid.org/0009-0007-1690-7191, Degens, H, Turner, MD ORCID: https://orcid.org/0000-0001-7175-1053, Stewart, C, Sale, C and Santos, L ORCID: https://orcid.org/0000-0002-1915-6780, 2023. Modelling skeletal muscle ageing and repair in vitro. Journal of Tissue Engineering and Regenerative Medicine, 2023: 9802235. ISSN 1932-6254

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Abstract

Healthy skeletal muscle can regenerate after ischaemic, mechanical, or toxin-induced injury, but ageing impairs that regeneration potential. This has been largely attributed to dysfunctional satellite cells and reduced myogenic capacity. Understanding which signalling pathways are associated with reduced myogenesis and impaired muscle regeneration can provide valuable information about the mechanisms driving muscle ageing and prompt the development of new therapies. To investigate this, we developed a high-throughput in vitro model to assess muscle regeneration in chemically injured C2C12 and human myotube-derived young and aged myoblast cultures. We observed a reduced regeneration capacity of aged cells, as indicated by an attenuated recovery towards preinjury myotube size and myogenic fusion index at the end of the regeneration period, in comparison with younger muscle cells that were fully recovered. RNA-sequencing data showed significant enrichment of KEGG signalling pathways, PI3K-Akt, and downregulation of GO processes associated with muscle development, differentiation, and contraction in aged but not in young muscle cells. Data presented here suggest that repair in response to in vitro injury is impaired in aged vs. young muscle cells. Our study establishes a framework that enables further understanding of the factors underlying impaired muscle regeneration in older age.

Item Type:	Journal article
Publication Title:	Journal of Tissue Engineering and Regenerative Medicine
Creators:	Tarum, J., Degens, H., Turner, M.D., Stewart, C., Sale, C. and Santos, L.
Contributors:	Name Role NTU ID ORCID Kuo, C.K. Editor UNSPECIFIED UNSPECIFIED
Publisher:	Hindawi Limited
Date:	4 July 2023
Volume:	2023
ISSN:	1932-6254
Identifiers:	Number Type 10.1155/2023/9802235 DOI 1779497 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jeremy Silvester
Date Added:	07 Jul 2023 14:27
Last Modified:	07 Jul 2023 14:29
URI:	https://irep.ntu.ac.uk/id/eprint/49336

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