Mitophagy suppression by miquelianin‐rich lotus leaves extract induces “beiging” of white fat viaAMPK/DRP1‐PINK1/PARKIN signaling axis

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Wang, Z, Yang, T, Zeng, M, Wang, Z, Chen, Q, Chen, J, Christian, M ORCID: https://orcid.org/0000-0002-1616-4179 and He, Z, 2023. Mitophagy suppression by miquelianin‐rich lotus leaves extract induces “beiging” of white fat viaAMPK/DRP1‐PINK1/PARKIN signaling axis. Journal of the Science of Food and Agriculture. ISSN 0022-5142

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Official URL: https://doi.org/10.1002/jsfa.13143

Abstract

Background: Lotus (Nelumbo nucifera) leaf has been described to have anti-obesity activity, but the role of white fat “browning” or “beiging” in its beneficial metabolic actions remains unclear. Here, 3T3-L1 cells and high-fat-diet (HFD)-fed mice were used to evaluate the effects of miquelianin-rich lotus leaf extract (LLE) on white-to-beige fat conversion and its regulatory mechanisms.

Results: Treatment with LLE increased mitochondrial abundance, mitochondrial membrane potential, and NAD+/NADH ratio in 3T3-L1 cells, suggesting its potential in promoting mitochondrial activity. qPCR and/or Western blotting analysis confirmed that LLE induced the expression of beige fat-enriched gene signatures (e.g., Sirt1, Cidea, Dio2, Prdm16, Ucp1, Cd40, Cd137, Cited1) and mitochondrial biogenesis-related markers (e.g., Nrf1, Cox2, Cox7a, Tfam) in 3T3-L1 cells and inguinal white adipose tissue (iWAT) of HFD-fed mice. Furthermore, we found that LLE treatment inhibited mitochondrial fission protein DRP1 and blocked mitophagy markers such as PINK1, PARKIN, BECLIN1, and LC-3B. Chemical inhibition experiments revealed that AMPK/DRP1 signaling was required for LLE-induced beige fat formation via suppressing PINK1/PARKIN/mitophagy.

Conclusion: Our data reveal a novel mechanism underlying the anti-obesity effect of LLE, namely the induction of white fat beiging via AMPK/DRP1/mitophagy signaling.

Item Type:	Journal article
Publication Title:	Journal of the Science of Food and Agriculture
Creators:	Wang, Z., Yang, T., Zeng, M., Wang, Z., Chen, Q., Chen, J., Christian, M. and He, Z.
Publisher:	Wiley
Date:	22 November 2023
ISSN:	0022-5142
Identifiers:	Number Type 10.1002/jsfa.13143 DOI 1839114 Other
Rights:	This is the peer reviewed version of the following article: WANG, Z., YANG, T., ZENG, M., WANG, Z., CHEN, Q., CHEN, J., CHRISTIAN, M. and HE, Z., 2023. Mitophagy suppression by miquelianin‐rich lotus leaves extract induces “beiging” of white fat viaAMPK/DRP1‐PINK1/PARKIN signaling axis. Journal of the Science of Food and Agriculture, which has been published in final form at https://doi.org/10.1002/jsfa.13143. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	29 Nov 2023 10:14
Last Modified:	22 Nov 2024 03:00
URI:	https://irep.ntu.ac.uk/id/eprint/50468