Microarray molecular mapping of horses with severe asthma

White, SJ ORCID: https://orcid.org/0000-0002-3675-7545, Couetil, L, Richard, EA, Marti, E and Wilson, PB ORCID: https://orcid.org/0000-0003-0207-2246, 2024. Microarray molecular mapping of horses with severe asthma. Journal of Veterinary Internal Medicine, 38 (1), pp. 477-484. ISSN 0891-6640

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Abstract

Background: Severe asthma (SA) in horses, resembling human asthma, is a prevalent, debilitating allergic respiratory condition marked by elevated allergen-specific immunoglobulin E (IgE) against environmental proteins; however, research exploring the exposome's influence on IgE profiles is currently limited but holds paramount significance for diagnostic and therapeutic developments.

Animals: Thirty-five sports horses were analyzed, consisting of environmentally matched samples from France (5 SA; 6 control), the United States (6 SA; 6 control), and Canada (6 SEA; 6 control).

Methods: This intentional cross-sectional study investigated the sensitization profiles of SA-affected and healthy horses via serological antigen microarray profiling. Partial least square-discriminant analysis (PLS-DA) was used to identify and rank the importance of allergens for class separation (ie, affected/non-affected) as variable influence of projection (VIP), and allergen with commonality internationally established via frequency analysis.

Results: PLS-DA models showed high discriminatory power in predicting SA in horses from Canada (area under the curve [AUC] 0.995) and France (AUC 0.867) but poor discriminatory power in horses from the United States (AUC 0.38). Hev b 5.0101, Cyn D, Der p 2, and Rum cr were the only shared allergens across all geographical groups.

Conclusions and Clinical Importance: Microarray profiling can identify specific allergenic components associated with SA in horses, while mathematical modeling of this data can be used for disease classification, highlighting the variability of sensitization profiles between geographical locations and emphasizing the importance of local exposure to the prevalence of different allergens. Frequency scoring analysis can identify important variables that contribute to the classification of SA across different geographical regions.

Item Type:	Journal article
Publication Title:	Journal of Veterinary Internal Medicine
Creators:	White, S.J., Couetil, L., Richard, E.A., Marti, E. and Wilson, P.B.
Publisher:	Wiley
Date:	2024
Volume:	38
Number:	1
ISSN:	0891-6640
Identifiers:	Number Type 10.1111/jvim.16951 DOI 1846351 Other
Rights:	This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Divisions:	Schools > School of Animal, Rural and Environmental Sciences Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	29 Jan 2024 13:52
Last Modified:	29 Jan 2024 13:52
URI:	https://irep.ntu.ac.uk/id/eprint/50760

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