The chemotrophic behaviour of Aspergillus niger: mapping hyphal filaments during chemo-sensing; the first step towards directed materials formation

Sadaf, A ORCID: https://orcid.org/0000-0003-1428-5783, Brock, M and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2024. The chemotrophic behaviour of Aspergillus niger: mapping hyphal filaments during chemo-sensing; the first step towards directed materials formation. Fungal Biology, 128 (4), pp. 1868-1875. ISSN 1878-6146

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Abstract

In the development of fungal based materials for applications in construction through to biomedical materials and fashion, understanding how to regulate and direct growth is key for gaining control over the form of material generated. Here, we show how simple ‘chemical food’ cues can be used to manipulate the growth of fungal networks by taking Aspergillus niger as an exemplar species. Chemotrophic responses towards a range of nitrogen and carbon containing biomolecules including amino acids, sugars and sugar alcohols were quantified in terms of chemotrophic index (CI) under a range of basal media compositions (low and high concentrations of N and C sources). Growth of filamentous networks was followed using fluorescence microscopy at single time points and during growth by an AI analytical approach to explore chemo sensing behaviour of the fungus when exposed to pairs (C-C, C-N, N-N) of biomolecules simultaneously. Data suggests that the directive growth of A. niger can be controlled towards simple biomolecules with CI values giving a good approximation for expected growth under a range of growth conditions. This is a first step towards identifying conditions for researcher-led directed growth of hyphae to make mycelial mats with tuneable morphological, physicochemical, and mechanical characteristics.

Item Type:	Journal article
Publication Title:	Fungal Biology
Creators:	Sadaf, A., Brock, M. and Perry, C.C.
Publisher:	Elsevier
Date:	June 2024
Volume:	128
Number:	4
ISSN:	1878-6146
Identifiers:	Number Type 10.1016/j.funbio.2024.05.010 DOI S1878614624000692 Publisher Item Identifier 1899246 Other
Rights:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	03 Jun 2024 08:22
Last Modified:	03 Jun 2024 08:22
URI:	https://irep.ntu.ac.uk/id/eprint/51507

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