Assessment of a light-curable hydrogel to be used for root canal obturation

Bhandari, S, Kuehne, S ORCID: https://orcid.org/0000-0001-6790-8433 and Camilleri, J, 2024. Assessment of a light-curable hydrogel to be used for root canal obturation. Journal of Dental Research. ISSN 0022-0345 (Forthcoming)

Text 2240568_Kuehne.pdf - Post-print Restricted to Repository staff only Download (5MB)

Abstract

Root canal obturation involves filling of the chemomechanically prepared root canal space. Despite reduced microbial load, residual bacteria can still lead to reinfection and treatment failure. Currently, obturation techniques use a combination of gutta-percha and sealer, which requires the preparation of the root canal to specific sizes and tapers to enable the fitting of customized cones. This study aims to characterize the physical, chemical, and antimicrobial properties of a new light-curable injectable material (OdneFill, Switzerland) used to obturate the root canal. Odnefill and 2 root canal sealers (AH Plus and BioRoot RCS) were characterized by scanning electron microscopy (SEM) and energy-dispersive spectroscopy following exposure to chlorhexidine, sodium hypochlorite, and water. The flow, film thickness, radiopacity, solubility, and contact angle were evaluated. The susceptibility to microbial degradation was assessed by weight changes after contact with bacterial enzymes (lipase and cholesterol esterase). A multispecies biofilm composed of Streptococcus mutans, Enterococcus faecalis, Fusobacterium nucleatum, and Veillonella dispar was used to assess changes to the material microstructure (SEM). Further, bacterial viability in contact with the materials was evaluated using live/dead staining and confocal microscopy. A direct contact assay was carried out, and the utilization of the materials as a carbon source for the bacterial biofilm was also assessed. Statistical analysis was performed using 1-way analysis of variance and Tukey post hoc tests (P = 0.05). OdneFill was composed of an organic matrix with zirconium oxide filler. It exhibited comparable physical properties to AH Plus and BioRoot RCS and was stable in contact with irrigating solutions and with the bacterial enzymes (cholesterol esterase and lipase). Its antimicrobial characteristics were better than those of AH Plus when placed in contact with a multispecies biofilm. Based on the findings, OdneFill presents itself as suitable root canal–filling material and warrants further clinical investigation.

Item Type:	Journal article
Publication Title:	Journal of Dental Research
Creators:	Bhandari, S., Kuehne, S. and Camilleri, J.
Publisher:	SAGE Publications
Date:	11 September 2024
ISSN:	0022-0345
Identifiers:	Number Type 10.1177/00220345241287504 DOI 2240568 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Ward
Date Added:	09 Oct 2024 12:38
Last Modified:	09 Oct 2024 12:38
URI:	https://irep.ntu.ac.uk/id/eprint/52382

Actions (login required)

Edit View

Statistics

Views

Downloads